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首页> 外文期刊>Drug delivery. >In vivo study of effects of artesunate nanoliposomes on human hepatocellular carcinoma xenografts in nude mice
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In vivo study of effects of artesunate nanoliposomes on human hepatocellular carcinoma xenografts in nude mice

机译：青蒿琥酯纳米脂质体对裸鼠人肝癌异种移植物影响的体内研究

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To investigate the effect of artesunate nanoliposomes on cultured cells in vitro and hepatocellular carcinoma xenografts in BALB/c-nu mice. Fluorescence polarization was applied for measurement of mitochondrial membrane fluidities; inhibition test of tumor cell proliferation in vitro was performed and nude mice xenograft model from human hepatocellular carcinoma (HCC) was established. Cytotoxicity of these compounds was evaluated by MTT assay on hepatocellular carcinoma xenografts in nude mice. Anisotropy (r-value) of blank nanoliposomes didn’t change, it had no statistically significance between the blank nanoliposomes group and the control group, it indicated that artesunate had no obvious effect on L-O2 human normal liver cells. IC50 values of artesunate nanoliposomes and artesunate API (active pharmaceutical ingredient) against HepG-2 cells were 15.997 and 19.706?μg/ml; IC50 values of the same drugs against L-O2 normal human liver cells were 100.23 and 105.54?μg/ml, respectively. Tumor growth inhibitory effect of artesunate nanoliposomes was 32.7%, and artesunate API was 20.5%, respectively. HepG-2 cells treated with artesunate nanoliposomes showed dose-dependent apoptosis. The antitumor effect of artesunate nanoliposomes on human hepatoma HepG2 cells were stronger than that of artesunate API at the same concentration.
机译：目的研究青蒿琥酯纳米脂质体对BALB / c-nu小鼠体外培养细胞和肝癌异种移植物的影响。荧光偏振用于测量线粒体膜的流动性。进行体外肿瘤细胞增殖抑制试验，建立人肝癌裸鼠异种移植模型。通过MTT测定对裸鼠肝细胞癌异种移植物评估这些化合物的细胞毒性。空白纳米脂质体的各向异性（r值）没有变化，在空白纳米脂质体组和对照组之间无统计学意义，表明青蒿琥酯对L-O2人正常肝细胞无明显作用。青蒿琥酯纳米脂质体和青蒿琥酯API（活性药物成分）对HepG-2细胞的IC 50 值为15.997和19.706？μg/ ml。相同药物对L-O2正常人肝细胞的IC 50 值分别为100.23和105.54？μg/ ml。青蒿琥酯纳米脂质体的肿瘤生长抑制作用分别为32.7％和青蒿琥酯API为20.5％。青蒿琥酯纳米脂质体处理的HepG-2细胞显示出剂量依赖性凋亡。在相同浓度下，青蒿琥酯纳米脂质体对人肝癌HepG2细胞的抗肿瘤作用强于青蒿琥酯API。

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