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Effect of live Salmonella Ty21a in Dextran Sulfate Sodium-induced Colitis

机译：活沙门氏菌Ty21a在硫酸右旋糖酐钠诱发的结肠炎中的作用

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Background: Intestinal microbiota seems to play an essential role in the development of inflammatory bowel diseases (IBD). We hypothesised that an oral vaccine based on live Salmonella typhi would be well tolerated and could even attenuate dextran sulfate sodium (DSS) induced colitis in rats, an animal model of IBD.Methods: Nine male Wistar rats was used for an initial tolerance study, in which we used 3 dose-levels of Salmonella Ty21a, 0.5 × 109, 1 × 109, and 2 × 109CFU, each dose being tested in 3 rats. Four treatment groups consisting of 8 male Wistar rats per group: 1) control group given standard food and water, 2) control group given four daily administrations of Salmonella Ty21a 1 × 109 CFU, 3) water with 5% DSS the last 7 days, 4) four daily administrations of Salmonella Ty21a before water with 5% DSS the last 7 days. The Salmonella Ty21a was administered by gastric gavage on day 1, 3, 5 and 16, while DSS was given with the drinking water from day 15 to 22. The animals were sacrificed and colonic tissue removed for analysis 22 days after gavage of the first vaccine dose.Results: The animals in the tolerance study got no signs of disease. In the treatment study, all animals receiving DSS had histologic indications of colitis, particularly in the distal part of the colon. Administration of Salmonella Ty21a had no significant effect on crypt and inflammation scores (p > 0.05).Conclusion: Gastric administration of live vaccine strain Salmonella Ty21a was well tolerated, but did not provide any significant protection against development of DSS induced colitis in rats.
机译：背景：肠道菌群似乎在炎症性肠病（IBD）的发展中起着至关重要的作用。我们假设基于活伤寒沙门氏菌的口服疫苗具有良好的耐受性，甚至可以减轻大鼠右旋糖酐硫酸钠（DSS）引起的结肠炎（IBD的动物模型）。方法：将9只雄性Wistar大鼠用于初始耐受性研究，其中我们使用了3种剂量水平的沙门氏菌Ty21a，0.5×109、1×109和2×109CFU，每种剂量均在3只大鼠中进行了测试。四个治疗组，每组8只雄性Wistar大鼠：1）对照组，给予标准食物和水，2）对照组，每天四次给予沙门氏菌Ty21a 1×109 CFU，3）最近7天加5％DSS的水， 4）在最后7天中，在每天用5％DSS浇水之前，每天进行四次沙门氏菌Ty21a施用。在第1、3、5和16天通过胃管饲喂沙门氏菌Ty21a，从第15天到22天用饮用水给予DSS。处死第一种疫苗后22天，处死动物并取出结肠组织进行分析结果：在耐受性研究中的动物没有疾病迹象。在治疗研究中，所有接受DSS的动物都有结肠炎的组织学迹象，特别是在结肠的远端。沙门氏菌Ty21a的施用对隐窝和炎症评分无显着影响（p> 0.05）。结论：胃用活疫苗菌株沙门氏菌Ty21a的耐受性良好，但未对DSS诱导的结肠炎大鼠产生任何明显的保护作用。

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