• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Jordan Journal of Biological Sciences >Expression of Biotransformation and Antioxidant Genes in the Liver of Albino Mice after Exposure to Aflatoxin B1 and an Antioxidant Sourced from Turmeric (Curcuma longa)
【24h】

Expression of Biotransformation and Antioxidant Genes in the Liver of Albino Mice after Exposure to Aflatoxin B1 and an Antioxidant Sourced from Turmeric (Curcuma longa)

机译：暴露于黄曲霉毒素B1和姜黄来源的抗氧化剂后，白化小鼠肝脏中生物转化和抗氧化基因的表达

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The present investigation aims to determine the effects of aflatoxin B1 (AFB1) on biotransformation and antioxidant genes and the protective effects of curcumin, present in turmeric (Curcuma longa) powder (TMP). Specifically, the study included four groups of albino mice were fed for 30 days on diet Group I: Control, Group II: animals fed on the conventional basal diet supplemented with 0.5% food grade TMP that supplied 74 mg/kg total curcuminoids. Group III contained animals reared on conventional basal diet supplemented with 1.0 ppm AFB1 supplied by ground aflatoxin culture material (760 ppm AFB1). Finally, Group IV comprised of albino mice fed with basal diet supplemented with 1.0 ppm AFB1 and 0.5% TMP that supplied 74 mg/kg of the body weight. After treatment, a number of physical parameters were assessed (gain in body weight, average quantity of feed intake and relative liver weight) and it was found that the subjects fed on diet containing curcumin and aflatoxin B1experienced ameliorative effect on the impact of aflatoxin B1 and performed better on growth and liver weight parameters. RNA extracted from the mice liver successfully was subjected to quantitative real time PCR analysis (Q PCR) and the results revealed no high significant difference in the expression of CAT gene between studied groups with probability value ≥0.005. However, at the other hand a decreased in expression statically of SOD, GPx, GST, EH genes was observed while there was an increased Synchronous and consistent expression of CYP1A1 and CYP2H1 genes display in the studied groups in the current study.
机译：本研究旨在确定黄曲霉毒素B1（AFB1）对姜黄粉（TMP）中生物转化和抗氧化剂基因的作用以及姜黄素的保护作用。具体而言，该研究包括四组白化病小鼠，以饮食喂养30天。组I：对照组，组II：以常规基础饮食喂养的动物，该饮食补充了0.5％食品级TMP，可提供74 mg / kg的总姜黄素。第三组包含按常规基础饮食饲养的动物，该饮食补充了由地面黄曲霉毒素培养物（760 ppm AFB1）提供的1.0 ppm AFB1。最后，第四组包括白化病小鼠，喂以补充了1.0 ppm AFB1和0.5％TMP的基础饮食，可提供74 mg / kg的体重。治疗后，评估了许多物理参数（体重增加，平均采食量和相对肝脏重量），发现以含姜黄素和黄曲霉毒素B1的饮食喂养的受试者对黄曲霉毒素B1和B2的影响有改善作用。在生长和肝脏重量参数方面表现更好。成功从小鼠肝脏中提取的RNA进行了定量实时PCR分析（Q PCR），结果显示，研究组之间CAT基因的表达没有很高的显着性，概率值≥0.005。然而，另一方面，在本研究中，观察到SOD，GPx，GST，EH基因的静态表达下降，而CYP1A1和CYP2H1基因的同步一致表达则增加。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号