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首页> 外文期刊>Journal of animal and veterinary advances >Characterisation of In vitro Gut Fermentation Products and their Metabolites from Corn Oil as Possible Source of Boldenone in Calves Nutrition
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Characterisation of In vitro Gut Fermentation Products and their Metabolites from Corn Oil as Possible Source of Boldenone in Calves Nutrition

机译：犊牛营养中肠内发酵产物及其来自玉米油的代谢产物可能是宝登酮的来源

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摘要

Phytosterols have been recently proposed as nutraceutics both in human and animal nutrition, because their influence on lipids metabolism. The reported possibility that phytosterols microbial fermentation could lead to the production of boldione as boldenone pro-hormone, pulsed us to set up two in vitro experiments for the characterisation of phytosterols gut fermentation products and their hepatic metabolites, by LC-MS/MS. A Todd-Hewitt broth (10 ml), enriched with corn oil as natural dietary source of phytosterols, was inoculated with 100 mg of calf faeces and aliquots drawn after 24h, 48h and 72h of micro-aerobic incubation. After SPE extraction, LC-MS/MS analysis revealed the presence of Boldione in the 24h fraction at a concentration of 4 ?g , while alpha and beta Boldenone were present in equimolar ratio in the 48h fraction at 12 ng each. Nor Boldione neither Boldenone were detected at 72h . The ether extract of the 48h fermented broth was further submitted to microsomial calf liver incubation. A not described major metabolite in a isomeric form, with a m/z 285 as protonated molecule has been found and its structure proposed . Such metabolite could be useful to trace in urine the origin of boldenone as metabolite from an alimentary source.
机译：最近有人提出将植物甾醇用作人类和动物营养中的营养保健品，因为它们对脂质代谢的影响。据报道，植物甾醇的微生物发酵可能会导致产生以金盏花酮为激素的前体激素，这促使我们建立了两个体外实验，以通过LC-MS / MS表征植物甾醇的肠道发酵产物及其肝代谢产物。在经过24小时，48小时和72小时的微需氧培养后，向100毫升富含玉米油作为天然饮食植物甾醇的Todd-Hewitt肉汤接种100 mg小牛粪便和等分试样。 SPE萃取后，LC-MS / MS分析显示24h馏分中存在Boldione，浓度为4 µg，而48h馏分中的等摩尔比的α和βBoldenone分别以12 ng存在。在72h时也没有发现Boldenone。将48h发酵肉汤的乙醚提取物进一步送入小腿小牛肝脏孵育。尚未发现以m / z 285为质子化分子的异构形式的主要代谢物，并提出了其结构。此类代谢物可能有助于从尿中追踪大丁烯酮的起源，其为来自食物来源的代谢物。

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