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首页> 外文期刊>Journal of Allergy >Evaluation of a Novel Rapid Test System for the Detection of Allergic Sensitization to Timothy Grass Pollen against Established Laboratory Methods and Skin Prick Test
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Evaluation of a Novel Rapid Test System for the Detection of Allergic Sensitization to Timothy Grass Pollen against Established Laboratory Methods and Skin Prick Test

机译：针对已建立的实验室方法和皮肤点刺试验检测提摩西草花粉过敏的新型快速试验系统的评估

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Type I hypersensitivity is driven by allergen specific immunoglobulin E (sIgE) and thus sIgE represents a marker for modern allergy diagnosis. Recently, a rapid assay for the detection of sIgE, termed as (Allergy Lateral Flow Assay) ALFA, has been developed. The objective of our study is the evaluation of a scanner-based system for the semiquantitative interpretation of ALFA results. Agreement to Skin Prick Test (SPT, Allergopharma), ALLERG-O-LIQ System (Dr. Fooke), and ImmunoCAP (Phadia) was investigated using 50 sera tested for specific IgE to timothy grass pollen (g6). 35/50 sera were positive by SPT, ALLERG-O-LIQ, and ImmunoCAP. Excellent agreement was observed between ALFA results and SPT, ImmunoCAP, and ALLERG-O-LIQ. Area under the curve (AUC) values were found at 1.0, and 100% sensitivity and specificity was found versus all other methods. Visual- and scanner-based interpretation of the ALFA results revealed excellent agreement.
机译：I型超敏反应由过敏原特异性免疫球蛋白E（sIgE）驱动，因此sIgE代表了现代过敏诊断的标志物。近来，已经开发了一种用于检测sIgE的快速测定法，称为（过敏性横向流测定法）ALFA。我们研究的目的是对基于扫描仪的系统进行ALFA结果的半定量评估。使用50份血清对提摩西花粉（g6）的特异性IgE进行了试验，研究了皮肤点刺试验（SPT，Allergopharma），ALLERG-O-LIQ系统（Fooke博士）和ImmunoCAP（Phadia）的协议。 SPT，ALLERG-O-LIQ和ImmunoCAP阳性的血清为35/50。在ALFA结果与SPT，ImmunoCAP和ALLERG-O-LIQ之间观察到极好的一致性。与所有其他方法相比，曲线下面积（AUC）值为1.0，并且发现100％的灵敏度和特异性。基于视觉和扫描仪的ALFA结果解释显示了极好的一致性。

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