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首页> 外文期刊>Journal of Behavioral and Brain Science >Altered Neurogranin Phosphorylation and Protein Levels Are Associated with Anxiety- and Depression-Like Behaviors in Rats Following Forced Swim Stress
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Altered Neurogranin Phosphorylation and Protein Levels Are Associated with Anxiety- and Depression-Like Behaviors in Rats Following Forced Swim Stress

机译：强迫游泳应激后大鼠中神经质粒素磷酸化和蛋白质水平的改变与焦虑和抑郁样行为相关。

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Here we tested the hypothesis that stress-induced alterations in Neurogranin (Ng) synthesis and/ or utilization might underlie stress-related depression and anxiety. Rats were randomly divided into five conditions: chronic swim stress (CS), acute swim stress (AS), and three control groups. The CS group was exposed to daily swim stress (5 min/day) for 14 consecutive days, the AS group received a single swim stress, and control groups were maintained in a stress-free condition. Both before and after swim stress, rats were tested for body weight gain, open-field locomotor activity, and saccharine preference. Ng and phospho-Ng (P-Ng) levels in the hippocampus and prefrontal cortex were determined by Western blot analysis. Compared to controls, CS animals displayed significantly decreased body weight gain, ambulation, and saccharine intake, and increased grooming behavior. CS animals had decreased Ng levels in the hippocampus and prefrontal cortex. In CS animals, Ng levels were positively correlated with saccharine intake and ambulation, and inversely correlated with grooming behavior. Compared to controls, AS increased immobility behavior and P-Ng and Ng levels in the hippocampus and prefrontal cortex. In AS animals, immobility behavior was positively correlated with the P-Ng in the prefrontal cortex. Thus, CS and AS produced opposing effects on Ng and P-Ng levels in the hippocampus and prefrontal cortex. Low Ng levels in the hippocampus were associated with anhedonic behavior in CS animals, whereas high P-Ng levels in the prefrontal cortex were associated with anxiety-like behavior in AS animals. Thus, Ng dysfunction might contribute to the neural mechanisms underlying stress-induced depression and anxiety.
机译：在这里，我们测试了一种假设，即压力诱导的神经颗粒素（Ng）合成和/或利用率变化可能是与压力相关的抑郁和焦虑的基础。将大鼠随机分为五个条件：慢性游泳压力（CS），急性游泳压力（AS）和三个对照组。 CS组连续14天每天承受游泳压力（5分钟/天），AS组承受一次游泳压力，对照组保持无压力状态。在游泳压力之前和之后，都对大鼠进行了体重增加，开阔地运动能力和糖精偏好的测试。通过蛋白质印迹分析确定海马和前额叶皮层中的Ng和磷酸Ng（P-Ng）水平。与对照组相比，CS动物的体重增加，行走能力和糖精摄入量显着降低，并且修饰行为增加。 CS动物的海马和前额叶皮层Ng水平降低。在CS动物中，Ng水平与糖精的摄入和运动呈正相关，与修饰行为呈负相关。与对照组相比，AS可增加海马和前额叶皮层的固定行为以及P-Ng和Ng水平。在AS动物中，固定行为与前额叶皮层中的P-Ng正相关。因此，CS和AS对海马和前额叶皮层的Ng和P-Ng水平产生相反的影响。海马中低Ng水平与CS动物的快感行为有关，而前额叶皮层中高P-Ng水平与AS动物的焦虑样行为相关。因此，Ng功能障碍可能有助于压力诱发的抑郁和焦虑的神经机制。

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