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首页> 外文期刊>Journal of Biomarkers >Urinary Measurement of Neutrophil Gelatinase Associated Lipocalin and Kidney Injury Molecule-1 Helps Diagnose Acute Pyelonephritis in a Preclinical Model
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Urinary Measurement of Neutrophil Gelatinase Associated Lipocalin and Kidney Injury Molecule-1 Helps Diagnose Acute Pyelonephritis in a Preclinical Model

机译：尿液测定中性粒细胞明胶酶相关的脂质脂蛋白和肾损伤分子-1有助于在临床前模型中诊断急性肾盂肾炎

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Background. The study assessed whether measurement of urinary biomarkers of acute kidney injury could be helpful in diagnosing acute pyelonephritis and subsequent scarring. Method. Escherichia coli J96 (0.3 mL inoculum containing /mL) was directly injected into the renal cortex of 3-week-old female Sprague Dawley rats (), with saline substituted in a control group (). Following the injection, urine was collected 2, 7, 14, 28, and 42 days after injection. Urinary neutrophil gelatinase associated lipocalin (NGAL), kidney injury molecule-1 (Kim-1), and interleukin-18 were quantitatively measured using enzyme-linked immunosorbent assay (ELISA). The levels of the biomarkers were adjusted for creatinine. Time course changes within a group or between the groups were compared. Correlation analysis was performed to understand the relationship between urinary levels and histological scarring. Results. Significantly elevated urinary NGAL was evident at two and seven days after injection, and Kim-1 was elevated at two days after injection. Receiver operating characteristic analyses confirmed the sensitivity of these markers at these times. No urinary marker at acute stage of APN was correlated with the amount of future scarring, negating their predictive value. Conclusion. Urinary NGAL and Kim-1 could be helpful in diagnosing febrile urinary tract infection in children.
机译：背景。该研究评估了急性肾损伤的尿液生物标志物的测定是否有助于诊断急性肾盂肾炎和随后的瘢痕形成。方法。将大肠埃希氏菌J96（含0.3？mL接种物/ mL）直接注射到3周龄雌性Sprague Dawley大鼠（）的肾皮质中，而对照组则用生理盐水代替。注射后，在注射后第2、7、14、28和42天收集尿液。尿中性粒细胞明胶酶相关脂钙蛋白（NGAL），肾损伤分子1（Kim-1）和白细胞介素18的含量采用酶联免疫吸附法（ELISA）进行了定量测定。调整了肌酐的生物标志物水平。比较组内或组间的时程变化。进行相关分析以了解尿液水平与组织学瘢痕形成之间的关系。结果。注射后两天和第七天尿NGAL明显升高，而注射后两天Kim-1升高。接收器工作特性分析确认了这些标记在这些时间的敏感性。 APN急性期的尿液指标与未来瘢痕形成的数量无关，否定了其预测价值。结论。尿NGAL和Kim-1可能有助于诊断儿童的高热性尿路感染。

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