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首页> 外文期刊>Journal of Brachial Plexus and Peripheral Nerve Injury >Functional cooperation of of IL-1β and RGS4 in the brachial plexus avulsion mediated brain reorganization
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Functional cooperation of of IL-1β and RGS4 in the brachial plexus avulsion mediated brain reorganization

机译：IL-1β和RGS4在臂丛神经撕脱介导的脑重组中的功能合作

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BackgroundsThere is considerable evidence that central nervous system is continuously modulated by activity, behavior and skill acquisition. This study is to examine the reorganization in cortical and subcortical regions in response to brachial plexus avulsion.MethodsAdult C57BL/6 mice were divided into four groups: control, 1, 3 and 6 month of brachial plexus avulsion. IL-1β, IL-6 and RGS4 expression in cortex, brainstem and spinal cord were detected by BiostarM-140 s microarray and real-time PCR. RGS4 subcellular distribution and modulation were further analyzed by primary neuron culture and Western Blot.ResultsAfter 1, 3 and 6 months of brachial plexus avulsion, 49 (0 up, 49 down), 29 (17 up, 12 down), 13 (9 up, 4 down) genes in cerebral cortex, 40 (8 up, 32 down), 11 (7 up, 4 down), 137 (63 up, 74 down) in brainstem, 27 (14 up, 13 down), 33 (18 up, 15 down), 60 (29 up, 31 down) in spinal cord were identified. Among the regulated gene, IL-1β and IL-6 were sustainable enhanced in brain stem, while PKACβ and RGS4 were up-regulated throughout cerebral cortex, brainstem and spinal cord in 3 and 6 month of nerve injury. Intriguingly, subcellular distribution of RGS4 in above three regions was dependent on the functional correlation of PKA and IL-1β.ConclusionData herein indicated that brachial plexus avulsion could efficiently initiate and perpetuate the brain reorganization. Network involved IL-1β and RGS4 signaling might implicate in the re-establish and strengthening of the local circuits at the cortical and subcortical levels.
机译：背景有大量证据表明，中枢神经系统受到活动，行为和技能习得的持续调节。这项研究是为了检查响应臂臂丛撕脱的皮层和皮层下区域的重组。方法将成年C57BL / 6小鼠分为四组：对照组，臂丛神经撕脱1、3和6个月。用BiostarM-140芯片和实时荧光定量PCR检测皮层，脑干和脊髓中IL-1β，IL-6和RGS4的表达。结果通过臂丛神经撕脱术1、3和6个月后，RGS4的亚细胞分布和调节进一步分析。结果在臂丛神经撕脱1、3和6个月后，49（0向上，49向下），29（17向上，12向下），13（9向上） ，大脑皮层中的4个基因，40个（8个向上，32个向下），11个（7个向上，4个向下），137个（63个向上，74个向下）在脑干中，27个（14个向上，13个向下），33（18个向上，向下15个），脊髓中60个（向上29个，向下31个）。在受调节的基因中，在神经损伤的3个月和6个月中，脑干中的IL-1β和IL-6持续增强，而PKACβ和RGS4在整个大脑皮层，脑干和脊髓中均上调。有趣的是，RGS4在上述三个区域中的亚细胞分布取决于PKA和IL-1β的功能相关性。结论本文的数据表明臂丛神经撕脱可以有效地引发和维持大脑的重组。涉及网络的IL-1β和RGS4信号可能暗示皮层和皮层下水平的局部回路的重建和加强。

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