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首页> 外文期刊>Journal of Botany >Nucleotide-Dependent Reprogramming of mRNAs Encoding Acetyl Coenzyme A Carboxylase and Lipoxygenase in Relation to the Fat Contents of Peanut
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Nucleotide-Dependent Reprogramming of mRNAs Encoding Acetyl Coenzyme A Carboxylase and Lipoxygenase in Relation to the Fat Contents of Peanut

机译：与花生脂肪含量相关的乙酰辅酶A羧化酶和脂氧合酶的mRNA的核苷酸依赖性重编程

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摘要

The nucleotide-dependent reprogramming of the mRNAs encoding acetyl coenzyme A carboxylase (ACC, EC 6.4.1.2) and lipoxygenase (LO, EC 1.13.11.12) was studied by Northern analysis. Equal concentrations of total RNA from the peanut (Arachis hypogaeaL. cv. “Valencia”) seedlings were probed with those glutamate dehydrogenase (GDH)-synthesized RNAs that were homologous to the mRNAs encoding ACC and LO. Treatment (4NTPs solution) of the peanuts increased the abundance of the mRNAs encoding the LO and ACC about 5 and 10 folds respectively but decreased the fat accumulation by 37% compared with the control peanut. Treatments (pyrimidine NTPs)of the peanut induced up to 60% more fat accumulation than the control. The high fat (40.0–44.0%) contents of the UTP-, and 3NTP-treated peanuts were characterized by very high abundance of the mRNA for ACC, but low abundance of the mRNA for LO. The inverse relationship between the abundance of the mRNAs encoding ACC and LO compared with the seedlings fat contents was due to the plus/minus strands sequence homologies between the Northern probes for ACC and LO. Therefore, LO, ACC, and the GDH-synthesized RNAs participated in the regulation of the fat contents. These results could be useful in the environmental manipulation of the quantity and quality of peanut oil.
机译：通过Northern分析研究了编码乙酰辅酶A羧化酶（ACC，EC 6.4.1.2）和脂氧合酶（LO，EC 1.13.11.12）的mRNA的核苷酸依赖性重编程。用那些与编码ACC和LO的mRNA同源的谷氨酸脱氢酶（GDH）合成的RNA，探查等浓度的花生（Arachis hypogaeaL。cv。“ Valencia”）的总RNA。花生处理（4NTPs溶液）与对照花生相比，编码LO和ACC的mRNA的丰度分别增加了约5倍和10倍，但脂肪堆积减少了37％。花生处理（嘧啶NTPs）诱导的脂肪积累比对照高60％。经UTP和3NTP处理的花生的高脂肪含量（40.0–44.0％），其特征是ACC的mRNA丰度很高，而LO的mRNA丰度却很低。与幼苗的脂肪含量相比，编码ACC和LO的mRNA丰度之间呈反比关系，这是由于ACC和LO的Northern探针之间的正负链序列同源性所致。因此，LO，ACC和GDH合成的RNA参与了脂肪含量的调节。这些结果可用于环境操作中花生油的数量和质量。

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