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首页> 外文期刊>Journal of Botany >Leaves and Seeds as Materials for Flow Cytometric Estimation of the Genome Size of 11 Rosaceae Woody Species Containing DNA-Staining Inhibitors
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Leaves and Seeds as Materials for Flow Cytometric Estimation of the Genome Size of 11 Rosaceae Woody Species Containing DNA-Staining Inhibitors

机译：叶子和种子作为流式细胞术估算11种含DNA抑制剂的酒渣鼻木本植物基因组大小的材料

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The presence of some secondary metabolites in the cell cytosol can cause a stoichiometric error in the flow cytometric estimation of nuclear DNA content. There is no fully reliable method to completely eliminate the effect of these compounds on nuclei fluorescence, and therefore using plant organs/parts free of staining inhibitors is recommended. Eleven species of Rosaceae with high concentrations of propidium-iodide-staining inhibitors were studied to check the possibility of using seeds instead of leaves for genome size estimation. Despite optimizing the concentration and composition of antioxidants in nuclei-isolation buffer for each species, the effect of cytosolic compounds present in the leaves could not be avoided entirely. None of the seeds of the studied species contained inhibitors, and they produced histograms of good quality. The genome size of the studied species ranged from 1.15 to 3.17 pg/2C; for 10 species the DNA content was estimated for the first time.
机译：细胞质溶胶中某些次生代谢产物的存在会在流式细胞术评估核DNA含量时引起化学计量误差。没有完全可靠的方法来完全消除这些化合物对细胞核荧光的影响，因此建议使用不含染色抑制剂的植物器官/部位。研究了11种高浓度碘化丙啶染色抑制剂的酒渣鼻，以检查使用种子代替叶子进行基因组大小估计的可能性。尽管针对每个物种优化了核隔离缓冲液中抗氧化剂的浓度和组成，但仍无法完全避免叶片中存在的胞质化合物的作用。所研究物种的种子均不含抑制剂，它们产生的直方图质量良好。所研究物种的基因组大小在1.15至3.17 pg / 2C之间。对于10个物种，DNA含量是首次估算。

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