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Endopolyploidy in Bryophytes: Widespread in Mosses and Absent in Liverworts

机译:苔藓植物的内源多倍体:在苔藓中广泛传播,在艾蒿中不存在

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Endopolyploidy occurs when DNA replication is not followed by mitotic nuclear division, resulting in tissues or organisms with nuclei of varying ploidy levels. Endopolyploidy appears to be a common phenomenon in plants, though the prevalence of endopolyploidy has not been determined in bryophytes (including mosses and liverworts). Forty moss species and six liverwort species were analyzed for the degree of endopolyploidy using flow cytometry. Nuclei were extracted in LB01 buffer and stained with propidium iodide. Of the forty moss species, all exhibited endopolyploid nuclei (mean cycle value=0.65±0.038) except for theSphagnummosses (mean cycle value=0). None of the liverwort species had endopolyploid nuclei (mean cycle value= 0.04 ± 0.014). As bryophytes form a paraphyletic grade leading to the tracheophytes, understanding the prevalence and role of endopolyploidy in this group is important.
机译:当DNA复制后没有有丝分裂的核分裂,就会发生内多倍体现象,从而导致组织或生物体的倍数水平发生变化。内胚多倍体似乎是植物中的常见现象,尽管尚未在苔藓植物(包括苔藓和艾蒿)中确定内多倍体的流行。使用流式细胞仪分析了40个苔藓种和6个艾蒿种的内多倍体程度。在LB01缓冲液中提取细胞核,并用碘化丙啶染色。在40种苔藓物种中,除鳞茎苔藓菌(平均周期值= 0)外,全部都显示内多倍体核(平均周期值= 0.65±0.038)。没有一个地艾属物种具有内多倍体核(平均周期值= 0.04±0.014)。由于苔藓植物形成了导致气管植物的副生植物等级,因此了解内多倍体的普遍性及其在这一组中的作用非常重要。

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