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首页> 外文期刊>Journal of Epithelial Biology & Pharmacology >Mineralocorticoid Receptor, Heat Shock Proteins and Immunophilins Participate in the Transmission of the Tubulogenic Signal of Aldosterone
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Mineralocorticoid Receptor, Heat Shock Proteins and Immunophilins Participate in the Transmission of the Tubulogenic Signal of Aldosterone

机译：盐皮质激素受体，热休克蛋白和免疫亲和素参与醛固酮的肾生成信号的传递。

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摘要

Aldosterone exhibits a tubulogenic activity on renal stem/progenitor cells. Little information is available about the signaling between the mineralocorticoid receptor (MR) and the proteins in the cytoplasm. To obtain information, renal stem/progenitor cells from neonatal rabbit were cultured at the interface of an artificial polyester interstitium in a perfusion container for 13 days. Development was registered with Soybean Agglutinin (SBA). Microscopy revealed that treatment with aldosterone (1 x 10-7 M) resulted in numerous SBA-labeled tubules. Interference with mineralocorticoid receptor (MR) and heat shock protein (hsp) 90 using geldanamycin (3,6 x 10-6 M) or radicicol (1 x 10-6 M) showed a lack of tubule development. Disturbing MR and hsp 70 with quercetin (2 x 10-4 M) and KNK 437 (1 x 10-4 M) generated SBAlabeled cell cluster but not structured tubules. Interference with MR and immunophilins by cyclosporin A (2 x 10-6 M) and rapamycin (2,2 x 10-9 M) did not influence the generation of numerous SBA-labeled tubules. In contrast, administration of FK 506 (2 x 10-8 M) resulted in a lack of tubule development. Thus, the signal for development of tubules induced by aldosterone is mediated by interaction of MR, hsp 90, hsp 70 and immunophilin FKBP 12.
机译：醛固酮对肾干/祖细胞表现出微管活性。关于盐皮质激素受体（MR）和细胞质中蛋白质之间信号传导的信息很少。为了获得信息，将新生兔的肾干/祖细胞在人工聚酯间质的界面在灌注容器中培养13天。开发已在大豆凝集素（SBA）中注册。显微镜检查显示，用醛固酮（1 x 10-7 M）治疗可导致大量SBA标记的肾小管。使用格尔德霉素（3.6 x 10-6 M）或radicicol（1 x 10-6 M）对盐皮质激素受体（MR）和热休克蛋白（hsp）90的干扰显示缺乏肾小管发育。用槲皮素（2 x 10-4 M）和KNK 437（1 x 10-4 M）干扰MR和hsp 70产生了SBA标记的细胞簇，但没有结构化的小管。环孢菌素A（2 x 10-6 M）和雷帕霉素（2,2 x 10-9 M）对MR和亲免素的干扰不影响许多SBA标记的小管的生成。相反，施用FK 506（2 x 10-8 M）导致缺乏肾小管发育。因此，醛固酮诱导的肾小管发育的信号是通过MR，hsp 90，hsp 70和亲免蛋白FKBP 12的相互作用介导的。

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