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Corrective Measures of Denaturing Gradient Gel Electrophoresis Limitations

机译:变性梯度凝胶电泳限制的校正措施

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Denaturing Gradient Gel Electrophoresis (DGGE) is a technique that attempts to monitor microbial populations, without culture dependant methods. This molecular biology approach is a fingerprinting methodology that revolutionary changes many of the traditional routines used in assessing microbial populations, PCR-DGGE is classified as part of the new discipline of molecular microbial ecology. Therefore, this study will briefly explain a number of applications that involve the technique and will also examine how DGGE works in general. In this study, various limitations of the DGGE technique and the corrective measures were briefly described that can improve the profitability, to get a real biodiversity.
机译:变性梯度凝胶电泳(DGGE)是一种尝试监测微生物种群而无需依赖培养物的方法的技术。这种分子生物学方法是一种指纹图谱方法,它彻底改变了用于评估微生物种群的许多传统程序,PCR-DGGE被列为分子微生物生态学新学科的一部分。因此,本研究将简要解释涉及该技术的许多应用,还将考察DGGE的总体工作原理。在这项研究中,简要介绍了DGGE技术的各种局限性和纠正措施,可以提高获利能力,以获得真正的生物多样性。

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