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Molecular Evidence for Determination Cryptic Species of Indonesian Swamp Eel Populations using Denaturing Gradient Gel Electrophoresis (DGGE)

机译:使用变性梯度凝胶电泳(DGGE)确定印度尼西亚沼泽鳗群的隐藏物种的分子证据(DGGE)

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Swamp eel is the Indonesian freshwater fish that should be understood and preserved due to their high economic potency in aquaculture. Additionally Indonesian swamp eel is commonly accepted to the species described as Monopterus albus (Synbranchiformes: Synbranchidae). However, the knowledge of genetic variation of this species from Indonesia is quite limited. Hence, in this study, we used the Denaturing Gradient Gel Electrophoresis (DGGE) and surveyed the genetic variation of cytochrome c oxidase subunit I (COI) in Indonesian swamp eels. Total of 300 samples of the swamp eel fish were collected from 15 populations in Indonesia. The DGGE results represented two genetic patterns (A and B) of the COI variations. In addition, the results revealed that five populations showed pattern A and six populations exhibited pattern B while four populations showed both patterns A and B of the COI variations. Therefore, the result supported the molecular data that the Indonesian swamp eel is a cryptic species complex. In addition, the distribution of swamp eel's COI patterns in Indonesia approves that DGGE is a rapid and sensitive method for the detection of a genetic variation of the fish.
机译:由于水产养殖的高经济效期,沼泽鳗鱼是印度尼西亚淡水鱼,应该被理解和保存。此外,印度尼西亚沼泽鳗通常被称为Monopterus Albus(Synbranchiformes:Synbranchidae)的物种。然而,从印度尼西亚的这种物种的遗传变异知识相当有限。因此,在本研究中,我们使用了变性梯度凝胶电泳(DGGE)并调查了印度尼西亚沼泽鳗中细胞色素C氧化酶亚基I(COI)的遗传变异。从印度尼西亚的15个种群中收集了300种沼泽鳗鱼鱼样品。 DGGE结果表示COI变化的两个遗传模式(A和B)。此外,结果表明,五个群体显示出图案A和六个种群表现出图案B,虽然四个群体显示了COI变化的图案A和B。因此,结果支持印度尼西亚沼泽鳗是隐秘物种复合物的分子数据。此外,印度尼西亚在印度尼西亚的COI模式的分布批准,DGGE是检测鱼的遗传变异的快速敏感的方法。

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