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首页> 外文期刊>Journal of genetics >Sequence variants of the LCORL gene and its association with growth and carcass traits in Qinchuan cattle in China
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Sequence variants of the LCORL gene and its association with growth and carcass traits in Qinchuan cattle in China

机译：中国秦川牛LCORL基因的序列变异及其与生长和s体性状的关系

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Molecular marker-assisted selection is a better way to satisfy the growing customer requirement with the development of beef cattle growth and breeding research. For now, quantitative trait locus (QTL) for cattle growth and carcass traits, just like body height, body length and carcass weight have been detected on bovine chromosome 6. In this study, ligand-dependent nuclear receptor corepressor-like (LCORL) was selected as the potential positional candidate gene located in chromosome 6 which is closely connected with the bovine growth and carcass traits. A total of 450 Qinchuan beef cattle were used to detect mutations in exon and its neighbouring region, and the promoter region of the bovine LCORL gene. The methods for SNPs detection werepolymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and created restriction site PCR (CRSPCR), and the results of this study show that there were two variations in intron regions, the other four variations were located in the promoter region. Linkage disequilibrium analysis and haplotype analysis indicated that L78-Q4 had strong linkage disequilibrium, A T G C G C (16.2%) and G C G C A T (16.7%) had higher haplotype frequencies, G C A C A C (0.8%) and G T A C A T (0.7%) had lower haplotype frequencies. Correlation analysis indicated that SNP g. INT+52098AG was significantly associated with slaughter weight and carcass weight. Based on the research, we selected LCORL as the candidate gene that can contribute to improved marker-assisted selection for the meat performance of Qinchuan beef cattle.
机译：随着肉牛生长和育种研究的发展，分子标记辅助选择是满足不断增长的客户需求的更好方法。到目前为止，已在牛第6号染色体上检测到了牛生长和cas体性状的定量性状基因座（QTL），就像身高，体长和car体重量一样。在这项研究中，配体依赖性核受体类似物（LCORL）是选择作为位于第6号染色体上的潜在位置候选基因，该基因与牛的生长和car体性状紧密相关。共有450头秦川肉牛用于检测外显子及其邻近区域和牛LCORL基因启动子区域的突变。 SNPs的检测方法为聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和创建限制性位点PCR（CRSPCR），研究结果表明内含子区域存在两个变异，另外四个位于内含子区域。启动子区域。连锁不平衡分析和单倍型分析表明，L78-Q4具有很强的连锁不平衡，A T G C G C（16.2％）和G C G C A T（16.7％）具有较高的单倍频率，G C A C A C（0.8％）和G T A C A T（0.7％）具有较低的单倍频率。相关分析表明SNP g。 INT + 52098A> G与屠宰体重和car体重量显着相关。根据这项研究，我们选择LCORL作为候选基因，可以帮助改进标记辅助选择来改善秦川肉牛的肉性能。

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