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首页> 外文期刊>Journal of genetics >Validation and dissection of quantitative trait loci for leaf traits in interval RM4923-RM402 on the short arm of rice chromosome 6
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Validation and dissection of quantitative trait loci for leaf traits in interval RM4923-RM402 on the short arm of rice chromosome 6

机译：水稻第6号染色体短臂上RM4923-RM402区间叶片性状的数量性状基因座的验证和解剖

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Validation and dissection of a QTL region for leaf traits in rice which has been reported in a number of independent studies were conducted. Three sets of near isogenic lines (NILs) were originated from a residual heterozygous line derived the indica cross Zhenshan 97B/Milyang 46. They were overlapping and totally covered a 4.2-Mb heterogenous region extending from RM4923 to RM402 on the short arm of rice chromosome 6. Each NIL set consisted of 10 maternal lines and 10 paternal lines. They were measured for the length, width, perimeter and area of the top three leaves and the number of spikelets per panicle, number of grains per panicle and grain weight per panicle. In NIL sets 6-4 and 6-7, differing in intervals RM4923-RM225 and RM19410-RM6119, respectively, significant variations with the enhancing alleles from the female parent ZS97 were shown for the length, perimeter and area except for the area of the third leaf from top in 6-4, but the effects were lower in 6-4 than in 6-7. No significant effects were detected for the three traits in the remaining NIL set. It was shown that flag leaf length (FLL) is the primary target of the QTLs detected. Two QTLs for FLL linked in repulsion phase were resolved, of which qFLL6.2 located in the 1.19-Mb interval RM3414-RM6917 had a major effect with the enhancing allele from Zhenshan 97B, and qFLL6.1 located in the 946.8-kb interval RM19350-RM19410 had a smaller effect with the enhancing allele from Milyang 46. The two QTLs also exerted pleiotropic effects on the yield traits.
机译：多项独立研究已报道了水稻叶性状的QTL区域的验证和解剖。三套近等基因系（NILs）来自于杂交后代Zhen山97B / Milyang 46的一个残留杂合子系。它们重叠并完全覆盖了水稻染色体短臂上一个从RM4923到RM402的4.2 Mb异质区域。 6.每组NIL由10条母系和10条父系组成。测量了它们的顶部三片叶子的长度，宽度，周长和面积，以及每穗的小穗数，每穗的谷粒数和每穗的粒重。在NIL组6-4和6-7中，分别的间隔RM4923-RM225和RM19410-RM6119不同，除雌性亲本ZS97的长度，周长和面积外，其他雌性ZS97的增强等位基因均显示出显着变化。从6-4的顶部倒数第三叶，但6-4的效果比6-7的低。在剩余的NIL组中，没有检测到这三个性状的显着影响。结果表明，旗叶长度（FLL）是检测到的QTL的主要目标。解决了两个在排斥期连锁的FLL的QTLs，其中位于1.19-Mb区间RM3414-RM6917的qFLL6.2对振山97B的增强等位基因有重要影响，而位于946.8kb区间RM19350的qFLL6.1具有重要作用。 -RM19410与来自Milyang 46的增强等位基因的作用较小。两个QTL对产量性状也具有多效性作用。

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