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首页> 外文期刊>Journal of inflammation. >The role of Qa-2, the functional homolog of HLA-G, in a Behcet's disease-like mouse model induced by the herpes virus simplex
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The role of Qa-2, the functional homolog of HLA-G, in a Behcet's disease-like mouse model induced by the herpes virus simplex

机译：Qa-2（HLA-G的功能同源物）在单纯疱疹病毒诱导的贝塞特病样小鼠模型中的作用

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Background It has been suggested that the HLA-G molecule is a genetic risk factor for Behcet's disease (BD). In this study, we evaluated the level of Qa-2, a murine nonclassical class I MHC molecule and possible functional homolog of HLA-G, to determine if it was associated with various symptoms of BD-like mice. In addition, we investigated siRNA (small interfering RNA) treatment to determine if it inhibited Qa-2 expression, thereby changing the symptoms of mice. Methods RNA interference (RNAi) and vector transfection were employed to manipulate gene expression in vivo in mice. siRNA (small interfering RNA) or Qa-2 expression vector was applied to inhibit or up-regulate Qa-2 expression, respectively. Results The Qa-2 levels in granulocytes were lower in BD-like mice than in normal controls. The silencing of Qa-2 by intravenous injection of siRNA (500 nmol/mouse, 4 times at 3-day intervals) specifically reduced the Qa-2 levels and worsened the BD-like symptoms. Conclusions Silencing Qa-2 by injecting siRNA into mice resulted in deterioration of symptoms in BD-like mice.
机译：背景技术已经提出，HLA-G分子是白塞氏病（BD）的遗传危险因素。在这项研究中，我们评估了鼠非经典I类MHC分子Qa-2的水平以及HLA-G的可能功能同源物，以确定其是否与BD样小鼠的各种症状有关。此外，我们研究了siRNA（小干扰RNA）治疗方法，以确定它是否抑制Qa-2表达，从而改变小鼠的症状。方法采用RNA干扰（RNAi）和载体转染的方法在小鼠体内进行基因表达调控。使用siRNA（小干扰RNA）或Qa-2表达载体分别抑制或上调Qa-2表达。结果BD样小鼠的粒细胞Qa-2水平低于正常对照组。通过静脉内注射siRNA（500 nmol /小鼠，每3天间隔4次）使Qa-2沉默，从而特别降低Qa-2的水平并加重BD样症状。结论通过向小鼠注射siRNA沉默Qa-2可导致BD样小鼠的症状恶化。

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