首页> 外文期刊>Journal of Insect Science >Segregation of European corn borer, Ostrinia nubilalis, aminopeptidase 1, cadherin, and bre5-like alleles, from a colony resistant to Bacillus thuringiensis Cry1Ab toxins, are not associated with F2 larval weights when fed a diet containing Cry1Ab
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Segregation of European corn borer, Ostrinia nubilalis, aminopeptidase 1, cadherin, and bre5-like alleles, from a colony resistant to Bacillus thuringiensis Cry1Ab toxins, are not associated with F2 larval weights when fed a diet containing Cry1Ab

机译:当饲喂含Cry1Ab的饮食时,欧洲玉米bore,玉米Ost,氨基肽酶1,钙粘蛋白和bre5等位基因与苏云金芽孢杆菌Cry1Ab毒素耐药菌落的分离与F2幼虫体重无关。

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Protein receptors may be required for activated Bacillus thuringiensis Cry toxins (Cry1Ab) to bind midgut epithelium prior to pore formation. Single nucleotide polymorphism markers from two Ostrinia nubilalis Hübner (Lepidoptera: Crambidae) midgut peptide receptors, cadherin (OnCad), aminopeptidase N 1 (OnAPN1), and OnBre5 (Onb3GalT5; a β-1,3-galactosyltransferase family 5 member) were used to examine segregation in F2 families derived from paired matings of Cry1Ab-resistant females and Cry1Ab-susceptible males. Genotypic frequencies for these markers did not deviate from Mendelian expectations. Analysis of F2 larvae indicate the segregation of single nucleotide pores in OnAPN1, OnBre5 (Onb3GalT5), and OnCad marker loci were independent of the segregation of log10 weights of larvae feeding on Cry1Ab diet.
机译:苏云金芽孢杆菌的Cry毒素(Cry1Ab)可能需要蛋白质受体才能在孔形成之前结合中肠上皮。使用了来自两个Ostrinia nubilalisHübner(鳞翅目:Crambidae)中肠肽受体,钙粘蛋白(OnCad),氨基肽酶N 1(OnAPN1)和OnBre5(Onb3GalT5;β-1,3-半乳糖基转移酶家族5成员)的单核苷酸多态性标记研究了由耐Cry1Ab的雌性和易受Cry1Ab的雄性成对交配的F 2 家族的隔离。这些标记的基因型频率没有偏离孟德尔的期望。对F 2 幼虫的分析表明,OnAPN1,OnBre5(Onb3GalT5)和OnCad标记基因座中的单核苷酸孔的分离与幼体喂养的log 10 重量的分离无关使用Cry1Ab饮食。

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