• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Invertebrate Pathology >Cross-resistance and mechanism of resistance to Cry1Ab toxin from Bacillus thuringiensis in a field-derived strain of European corn borer, Ostrinia nubilalis.
【24h】

Cross-resistance and mechanism of resistance to Cry1Ab toxin from Bacillus thuringiensis in a field-derived strain of European corn borer, Ostrinia nubilalis.

机译：苏云金芽孢杆菌Cry1Ab毒素在欧洲玉米bore田strain中的交叉抗性和抗性机理。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The cross-resistance spectrum and biochemical mechanism of resistance to the Bacillus thuringiensis Cry1Ab toxin was studied in a field-derived strain of Ostrinia nubilalis (Hubner) (Lepidoptera: Crambidae) that was further selected in the laboratory for high levels (>1000-fold) of resistance to Cry1Ab. The resistant strain exhibited high levels of cross-resistance to Cry1Ac and Cry1Aa but only low levels of cross-resistance (<4-fold) to Cry1F. In addition, there was no significant difference between the levels of resistance to full-length and trypsin-activated Cry1Ab protein. No differences in activity of luminal gut proteases or altered proteolytic processing of the toxin were observed in the resistant strain. Significantly reduced binding of radiolabeled Cry1Aa was observed in the resistant strain whereas binding of Cry1Ab and Cry1Ac was practically the same in both resistant and susceptible strains. The interpretation of the overall data seems to suggest the involvement of an alteration in the binding of Cry1A toxins to a common receptor, which is more clearly revealed by the binding assays using radiolabeled Cry1Aa.
机译：在田间衍生的 Ostrinia nubilalis （Hubner）（鳞翅目：Crambidae）的菌株中研究了对苏云金芽孢杆菌Cry1Ab毒素的抗性的交叉耐药谱和生化机理。在实验室中进一步选择Cry1Ab的高水平抗性（> 1000倍）。抗性菌株对Cry1Ac和Cry1Aa表现出高水平的交叉抗性，但对Cry1F表现出低水平的交叉抗性（<4倍）。此外，对全长和胰蛋白酶激活的Cry1Ab蛋白的耐药水平之间没有显着差异。在抗性菌株中未观察到腔肠蛋白酶的活性差异或毒素的蛋白水解过程改变。在耐药菌株中观察到放射性标记的Cry1Aa的结合显着降低，而在耐药菌株和易感菌株中Cry1Ab和Cry1Ac的结合实际上是相同的。总体数据的解释似乎暗示了Cry1A毒素与普通受体结合的改变，这通过使用放射性标记的Cry1Aa的结合测定更清楚地揭示出来。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号