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首页> 外文学位 >Characterization of resistance to the Cry1Ab toxin from Bacillus thuringiensis Berliner in a field-derived strain of European corn borer, Ostrinia nubilalis (Hubner) (Lepidoptera: Crambidae).
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Characterization of resistance to the Cry1Ab toxin from Bacillus thuringiensis Berliner in a field-derived strain of European corn borer, Ostrinia nubilalis (Hubner) (Lepidoptera: Crambidae).

机译：在来自欧洲玉米bore的田strain中，对苏云金芽孢杆菌Berliner的Cry1Ab毒素的抗性表征。

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摘要

The Cry1Ab toxin from Bacillus thuringiensis is expressed by 80% of the commercially available transgenic maize, Zea mays L., that targets the European corn borer, Ostrinia nubilalis (Hubner). The resistance management plan for O. nubilalis is based on the high dose refuge strategy and monitoring of field populations to detect resistance. We compared methods to quantify Cry1Ab toxin for standardization of insect bioassays. Standardization of Cry1Ab production and quantification should improve consistency in bioassay data to quantify resistance and to identify changes in susceptibility of O. nubilalis to Cry1Ab toxin. A field collection of O. nubilalis from Kandiyohi County, Minnesota exhibited significantly lower mortality at the diagnostic concentration developed for O. nubilalis. We used standardized bioassays, on-plant assays, life table analysis and biochemical studies to characterize resistance in insects from this field collection. The resistant strain developed high levels of resistance to Cry1Ab, variable levels of cross-resistance to other Cry toxins, and the ability to survive on transgenic corn by feeding on tissues of low Cry1Ab expression. Studies to determine the inheritance of resistance and its fitness costs revealed that resistance is most likely autosomal, incompletely recessive, and polygenic. The inheritance of fitness costs of resistance to Cry1Ab in O. nubilalis varied from incompletely recessive to recessive among the parameters evaluated using life table analysis. Cry1Ab binding to brush-border membrane vesicles (BBMV) of susceptible and resistant larvae was compared using surface plasmon resonance and ligand-toxin immunoblot analysis to test the hypothesis that resistance involves reduction of toxin binding to receptors. We also examined proteolytic degradation of Cry1Ab as a candidate mechanism of resistance. Although we found reduced Cry1Ab and Cry1Aa binding in BBMV of resistant insects that were consistent with the cross-resistance pattern observed in bioassays, the exact nature of the resistance remains uncertain.
机译：来自苏云金芽孢杆菌的Cry1Ab毒素由80％的以欧洲玉米bore为研究对象的玉米转基因玉米Zea mays L.表达。 O.nubilalis的抗药性管理计划基于高剂量避难所策略和对田间种群的监测以检测抗药性。我们比较了量化Cry1Ab毒素的昆虫生物测定方法的标准化方法。 Cry1Ab产生和定量的标准化应提高生物测定数据的一致性，以量化抗药性并鉴定脐橙对Cry1Ab毒素的敏感性变化。在明尼苏达州坎迪约希县的田野O. nubilalis田间采集的病原菌在诊断浓度下显着降低了死亡率。我们使用了标准化的生物测定，植物测定，生命表分析和生化研究来表征该田间采集的昆虫的抗性。该抗性菌株对Cry1Ab产生了高水平的抗性，对其他Cry毒素具有不同水平的交叉抗性，并通过以低Cry1Ab表达的组织为食而在转基因玉米上存活。确定抗性遗传及其适应性成本的研究表明，抗性很可能是常染色体的，不完全隐性的和多基因的。在使用生命表分析评估的参数中，对O. nubilalis的Cry1Ab抗性的适应性成本的继承从不完全隐性到隐性。使用表面等离振子共振和配体毒素免疫印迹分析比较了Cry1Ab与易感和抗性幼虫的刷状边界膜囊泡（BBMV）的结合，以检验抗性涉及减少毒素与受体结合的假设。我们还检查了Cry1Ab的蛋白水解降解作为抗药性的候选机制。尽管我们发现抗性昆虫的BBMV中Cry1Ab和Cry1Aa结合减少，这与生物测定中观察到的交叉耐药模式一致，但耐药性的确切性质仍然不确定。

著录项

  • 作者

    Crespo, Andre Luiz B.;

  • 作者单位

    The University of Nebraska - Lincoln.;

  • 授予单位 The University of Nebraska - Lincoln.;
  • 学科 Biology Entomology.
  • 学位 Ph.D.
  • 年度 2008
  • 页码 157 p.
  • 总页数 157
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 昆虫学;
  • 关键词

  • 入库时间 2022-08-17 11:38:49

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