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首页> 外文期刊>Pest Management Science >On-plant survival and inheritance of resistance to Cry 1 Ab toxin from Bacillus thuringiensis in a field-derived strain of European corn borer, Ostrinia nubilalis
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On-plant survival and inheritance of resistance to Cry 1 Ab toxin from Bacillus thuringiensis in a field-derived strain of European corn borer, Ostrinia nubilalis

机译：苏云金芽孢杆菌Cry 1 Ab毒素在田间衍生的欧洲玉米bore田中的存活率和抗性遗传

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摘要

BACKGROUND: The high dose plus refuge is one of the major components of the resistance management plan mandated for transgenic corn expressing Cry toxins from Bacillus thuringiensis Berliner (Bt) that targets the European corn borer, Ostrinia nubilalis (Hiibner) (Lepidoptera: Crambidae). This strategy was based on assumptions such as functional recessive inheritance, which has not been previously tested for O. nubilalis. The authors used a field-derived resistant strain of O. nubilalis to define the nature of resistance to Cry1Ab toxin by examining the inheritance and on-plant survival of susceptible and resistant insects and their F_1 progeny. RESULTS: The resistant strain exhibited > 800-fold resistance to Cry1Ab. Resistance was primarily autosomal and controlled by more than one locus or multiple alleles at one locus. The degree of dominance D calculated on the basis of LC_(50) values was-0.45 (h' = 0.27), indicating that resistance was incompletely recessive. No survivors were found on vegetative-stage Bt corn, although both resistant larvae and their F_1 progeny were able to survive on reproductive corn 15 days after infestation. CONCLUSIONS: A field derived O. nubilalis strain exhibited high levels of resistance to Cry1Ab and survived on transgenic corn by feeding on tissues with low Cry1Ab expression. The Cry1Ab resistance was primarily autosomal, incompletely recessive and polygenic. Tissue and on-plant survival data indicated that dominance varies depending on plant stage.
机译：背景：高剂量加避难所是抗性管理计划的主要组成部分之一，该计划授权用于表达来自苏云金芽孢杆菌柏林（Bt）的转基因玉米的Cry毒素，其针对欧洲玉米蛀虫Ostrinia nubilalis（Hiibner）（鳞翅目：Crambidae）。该策略基于诸如功能隐性遗传的假设，该假设之前尚未经过过O.nubilalis的测试。这组作者通过检查敏感和抗性昆虫及其F_1后代的遗传和在植物中的存活情况，使用了野外产的O. nubilalis抗性菌株来定义对Cry1Ab毒素的抗性性质。结果：该抗性菌株对Cry1Ab的抗性> 800倍。抗性主要是常染色体性的，并由一个位点以上的一个位点或多个等位基因控制。根据LC_（50）值计算出的优势度D为-0.45（h'= 0.27），表明阻力不完全是隐性的。在营养期的Bt玉米上没有发现任何存活者，尽管在侵染后15天，抗性幼虫及其F_1后代都能在繁殖玉米上存活。结论：野外产的O. nubilalis菌株对Cry1Ab表现出高水平的抗性，并通过以低Cry1Ab表达的组织为食而在转基因玉米上存活。 Cry1Ab耐药主要是常染色体，不完全隐性和多基因的。组织和植物上的存活数据表明，优势取决于植物阶段。

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