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首页> 外文期刊>Journal of King Saud University >Digestive α-amylase of Bacterocera oleae Gmelin (Diptera: Tephritidae): Biochemical characterization and effect of proteinaceous inhibitor
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Digestive α-amylase of Bacterocera oleae Gmelin (Diptera: Tephritidae): Biochemical characterization and effect of proteinaceous inhibitor

机译：油菜细菌 Gmelin（Diptera：Tephritidae）的消化α-淀粉酶：蛋白质抑制剂的生化特性和作用

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Digestive α-amylase of Bacterocera oleae larvae was characterized and treated by an inhibitor to gain a better understanding of the degradation of nutritional molecules as a potential target for controlling the pest. Presence of α-amylase was confirmed in the gut of olive fruit fly through the use of a negative control in dinitrosalicylic acid procedure. An optimal pH of 5 was found for amylolytic activity in the gut. The enzyme had optimal activity in a broad range of temperatures 20–45 °C. Among used cations and specific inhibitors, Ca 2+ , phenylmethylsulphonyl fluoride (PMSF) and ethylene glycol-bis (β-aminoethylether) N,N,N′,N′-tetraacetic acid (EGTA) had statistical differences on amylolytic activity indicating the presence of amino acid triad and Ca 2+ in active site of the enzyme. A proteinaceous α-amylase inhibitor was extracted from Polygonum persicaria , a medicinal plant, that widely grows in North of Iran. IC 50 value of PPAI was 0.062 mg/ml (i.e. 0.062 mg/ml of extracted inhibitor inhibited 50% of amylolytic activity in the gut of B. oleae larvae) and was temperature and pH dependent. The use of enzyme inhibitors from different plant sources may serve as an important pest control strategy via plant breeding programs. Identification of genes responsible for these inhibitor proteins could be a first step to provide a resistant variety of olive.
机译：对油酸细菌幼虫的消化α-淀粉酶进行了表征，并用抑制剂进行处理，以更好地了解营养分子的降解，将其作为控制害虫的潜在靶标。通过在二硝基水杨酸程序中使用阴性对照，确认了橄榄果蝇肠道中存在α-淀粉酶。发现肠道中的淀粉分解活性最佳pH为5。该酶在20–45°C的广泛温度范围内具有最佳活性。在使用过的阳离子和特定抑制剂中，Ca 2+，苯甲基磺酰氟（PMSF）和乙二醇双（β-氨基乙基醚）N，N，N'，N'-四乙酸（EGTA）在淀粉分解活性方面存在统计差异，表明存在酶活性位点中氨基酸三联体和Ca 2+的表达一种蛋白质α-淀粉酶抑制剂是从多年生于伊朗北部的药用植物何首乌（Polygonum persicaria）中提取的。 PPAI的IC 50值为0.062 mg / ml（即提取的抑制剂0.062 mg / ml抑制油菜双歧杆菌幼虫肠道中50％的淀粉分解活性），并且与温度和pH有关。通过植物育种计划，使用来自不同植物来源的酶抑制剂可作为重要的害虫防治策略。鉴定负责这些抑制剂蛋白的基因可能是提供抗性橄榄的第一步。

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