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首页> 外文期刊>Journal of King Saud University >In vitro free radical scavenging activities of aerial parts’ aqueous extract and extract fractions of Ampelocissus latifolia (Roxb.) Planch. in relation to total phenolics and flavonoid contents
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In vitro free radical scavenging activities of aerial parts’ aqueous extract and extract fractions of Ampelocissus latifolia (Roxb.) Planch. in relation to total phenolics and flavonoid contents

机译：体外清除自由基的水上提取物和 Ampelocissus latifolia （Roxb。）Planch馏分的自由基活性。与总酚和类黄酮含量有关

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Discovery of new antioxidants of plant origin is of current interest.Ampelocissus latifolia(Roxb.) Planch. is a traditionally used medicinal plant. The present study was aimed to investigate thein vitrofree radical scavenging activities of aerial parts’ aqueous extract ofA. latifolia(AAEAL) and its organic solvent extract fractions in relation to their total phenolics and flavonoid contents. The AAEAL was fractionated with the organic solvents like petroleum ether (AAEALPE), chloroform (AAEALCH), ethyl acetate (AAEALEA), and n-butanol (AAEALNB). For the determination of antioxidant activity of AAEAL and its extract fractions, Fe3+ion reducing antioxidant power (FRAP), 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, and total antioxidant assays were performed and the total phenolics and flavonoid contents were also estimated. The EC50of the ferric ion reduction by AAEALEA and ascorbic acid were calculated as 90.8?±?4.70 and 84.86?±?7.22?μg/mL respectively. The IC50of DPPH free radical scavenging with AAEALEA and ascorbic acid were calculated as 19.5?±?0.5 and 18.35?±?1.15?μg/mL respectively. The overall data indicate that the AAEALEA possesses the highest total antioxidant activity (37.85?±?1.55?μg ascorbic acid equivalents/100?μg dry extract matter), the highest total phenolics (65.89?±?1.33?mg tannic acid equivalent phenolics/100?mg dry extract) and flavonoid (30.67?±?0.28?mg quercetin equivalent flavonoids/100?mg dry extract) contents. The antioxidant activities were also positively correlated with the total phenolics and flavonoid contents of the extract fractions. In summary, AAEAL may be considered as a natural source of antioxidants and its ethyl acetate extract fraction seems to be equally effective to the ascorbic acid as an antioxidant.
机译：目前，新的植物来源抗氧化剂的发现引起了人们的兴趣。Ampelocissus latifolia（Roxb。）Planch是传统上使用的药用植物。本研究旨在研究地上部分A的水提物的体外清除自由基活性。阔叶茶（AAEAL）及其有机溶剂提取物馏分与总酚和类黄酮含量的关系。将AAEAL用有机溶剂（如石油醚（AAEALPE），氯仿（AAEALCH），乙酸乙酯（AAEALEA）和正丁醇（AAEALNB）进行分馏。为了测定AAEAL及其提取物部分的抗氧化活性，进行了Fe3 +离子还原抗氧化能力（FRAP），1，1-二苯基-2-吡啶并肼基（DPPH）自由基清除，总抗氧化剂测定以及总酚和还估计了类黄酮含量。 AAEALEA和抗坏血酸还原铁离子的EC50分别为90.8？±？4.70和84.86？±？7.22？g / mL。用AAEALEA和抗坏血酸清除DPPH自由基的IC50分别为19.5？±？0.5和18.35？±？1.15？g / mL。总体数据表明，AEAEALEA具有最高的总抗氧化剂活性（37.85？±？1.55？g抗坏血酸当量/ 100？μg干提取物），最高的总酚类（65.89？±？1.33？mg单宁酸当量酚/含量为100？mg干提取物）和类黄酮（相当于30.67？±？0.28？mg槲皮素当量类黄酮/ 100？mg干提取物）的含量。抗氧化活性也与提取物中的总酚和类黄酮含量呈正相关。总而言之，AAEAL可以被认为是抗氧化剂的天然来源，其乙酸乙酯提取物级分对于抗坏血酸的抗氧化作用似乎同样有效。

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