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首页> 外文期刊>Journal of King Saud University >Isolation and purification of starch hydrolysing amylase from Streptomyces sp . Al-Dhabi-46 obtained from the Jazan region of Saudi Arabia with industrial applications
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Isolation and purification of starch hydrolysing amylase from Streptomyces sp . Al-Dhabi-46 obtained from the Jazan region of Saudi Arabia with industrial applications

机译：从链霉菌 sp Al-Dhabi-46分离和纯化淀粉水解淀粉酶，该酶得自沙特阿拉伯的Jazan地区，具有工业用途

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Amylase is an important industrially useful enzyme with various applications, widely explored for their applications in food and biofuel industries. In this study, seventeen actinomycetes strains were isolated from the soil sample and screened for amylase biosynthesis. Among the 17 isolates, nine actinomycetes showed activity on starch agar plates. The strain Al-Dhabi-46 showed more hydrolytic zone (19?mm) on starch agar plates. It was identified by biochemical characters and 18S rDNA sequencing and characterized asStreptomycessp. Al-Dhabi-46. Amylase production was found to be maximum after 5?days of incubation (108?±?4.1?U/ml) and at pH 8.0 (118.1?±?2.3?U/ml) and at 40?°C (124?±?12.1?U/ml). Amylase synthesis was high in the medium containing starch (208?±?11.4?U/ml). Amylase production was 241?±?18.1?U/ml in the culture medium supplemented with beef extract and 0.1% Mn2+ions significantly enhanced amylase production. The molecular weight of purified amylase fromStreptomycessp. Al-Dhabi-46 was 44?kDa. Amylase was highly active up to pH 8.0 and at 40?°C. Amylase activity was enhanced by the addition of Mn2+at 10?mM concentrations. Purified amylase fromStreptomycessp. Al-Dhabi-46 digested cassava starch at 25% and 55%, 78% and 95.4% level after 15?min, 30?min, 45?min and 60?min of enzymatic reaction. Enhanced production of amylase enzyme from theStreptomycessp. Al-Dhabi-46 attract for the production of industrially important products.
机译：淀粉酶是一种重要的工业上有用的酶，具有多种应用，已广泛应用于食品和生物燃料行业。在这项研究中，从土壤样品中分离出十七种放线菌菌株，并筛选了淀粉酶的生物合成。在这17种分离物中，有9种放线菌在淀粉琼脂平板上显示出活性。 Al-Dhabi-46菌株在淀粉琼脂平板上显示出更多的水解区（19？mm）。通过生化特性和18S rDNA测序对其进行鉴定，并将其表征为链霉菌。 Al-Dhabi-46。孵育5天后（108？±？4.1？U / ml），pH 8.0（118.1？±？2.3？U / ml）和40？C（124？±？）时，淀粉酶的产量最高。 12.1？U / ml）。在含淀粉的培养基中，淀粉酶的合成很高（208±1±11.4μU/ ml）。在补充有牛肉提取物和0.1％Mn2 +离子的培养基中，淀粉酶的产量为241±±18.1U / ml。来自链霉菌属的纯化淀粉酶的分子量。 Al-Dhabi-46为44？kDa。淀粉酶在pH值8.0和40°C下都具有很高的活性。通过添加10？mM浓度的Mn2 +可以增强淀粉酶的活性。来自链霉菌属的纯化的淀粉酶。酶反应15分钟，30分钟，45分钟和60分钟后，Al-Dhabi-46消化的木薯淀粉含量分别为25％和55％，78％和95.4％。来自链霉菌的淀粉酶的产量提高。 Al-Dhabi-46吸引了重要工业产品的生产。

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