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首页> 外文期刊>Journal of Korean medical science. >Negative correlation between the conversion of thyrotropin receptor-bound blocking type thyrotropin receptor antibody to the stimulating type by anti-human IgG antibodies and the biological activity of blocking type thyrotropin receptor antibody
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Negative correlation between the conversion of thyrotropin receptor-bound blocking type thyrotropin receptor antibody to the stimulating type by anti-human IgG antibodies and the biological activity of blocking type thyrotropin receptor antibody

机译：抗人IgG抗体将结合促甲状腺激素受体的阻断型促甲状腺素受体抗体转化为刺激型与阻断型促甲状腺素受体抗体的生物学活性之间负相关

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摘要

It has been reported that receptor-bound blocking type TSH receptor antibody (TRAb) can be converted to the stimulating type by anti-human IgG antibodies. To evaluate the relationship between the conversion of receptor-bound blocking type TRAb to the stimulating type and the biological activity of blocking type TRAb, we compared converting activities of blocking type TRAb from 10 patients with primary nongoitrous hypothyroidism with both the doses of blocking type TRAb which show 50% inhibition of 125I-bTSH binding to the TSH receptor and those which show 50% inhibition of TSH-stimulated cAMP production in cultured rat thyroid cells (FRTL-5). The additions of anti-human IgG antibody to FRTL-5 cell-bound blocking IgGs resulted in the increase in cAMP production in a dose-dependent manner and the converting activities (percent increase of cAMP production) also depended on the doses of blocking IgGs. The converting activities were significantly correlated with the doses of blocking IgGs which showed 50% inhibition of 125I-bTSH binding to the TSH receptor (r = 0.71, p = 0.011). And these converting activities were also significantly correlated with the doses of blocking IgGs which showed 50% inhibition of TSH-stimulated cAMP increase (r = 0.81, p = 0.002), and were negatively correlated with thyroid stimulation blocking antibody activities (r = 0.58, p = 0.02). We have demonstrated that all cell-bound blocking type TRAb were converted to the stimulating type by anti-human IgG antibody and the degree of conversion was negatively correlated with the biological activity of blocking type TRAb.(ABSTRACT TRUNCATED AT 250 WORDS)
机译：据报道，受体结合的阻断型TSH受体抗体（TRAb）可以通过抗人IgG抗体转化为刺激型。为了评估受体结合的阻断型TRAb转化为刺激型与阻断型TRAb的生物学活性之间的关系，我们将10例原发性非甲状腺功能减退症患者的阻断型TRAb的转化活性与两种剂量的阻断型TRAb进行了比较它们在培养的大鼠甲状腺细胞（FRTL-5）中显示出125％抑制125I-bTSH与TSH受体的结合，而显示出50％抑制TSH刺激的cAMP产生。将抗人IgG抗体添加到FRTL-5细胞结合的封闭IgG中，导致cAMP产量以剂量依赖性方式增加，并且转化活性（cAMP产量的百分比增加）也取决于封闭IgG的剂量。转化活性与阻断IgG的剂量显着相关，后者显示125I-bTSH与TSH受体的结合受到50％的抑制（r = 0.71，p = 0.011）。并且这些转化活性还与阻断IgG的剂量显着相关，后者显示TSH刺激的cAMP升高受到50％的抑制（r = 0.81，p = 0.002），与甲状腺刺激阻断抗体活性呈负相关（r = 0.58， p = 0.02）。我们已经证明所有细胞结合的阻断型TRAb均被抗人IgG抗体转化为刺激型，并且转化程度与阻断型TRAb的生物学活性呈负相关。（摘要截短为250字）

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