...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Agricultural Science >Silicon in the Embriogenic Potential of Callus in vitro of Passiflora edulis
【24h】

Silicon in the Embriogenic Potential of Callus in vitro of Passiflora edulis

机译：硅在西番莲离体愈伤组织的胚发生潜能中

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Adding silicon to the culture medium may contribute, to improve many growth parameters including embryogenesis and organogenesis. The objective of this study was to evaluate the embryogenic potential of passion fruit in vitro (Passiflora edulis f. Flavicarpa O. Deg), submitted to different concentrations of silicon. Nodal segments of plants were inoculated at five concentrations of silicic acid added to the culture medium, 0.0; 0.5; 1.0; 1.5 and 2.0 g L-1. After 60 days, the visual characteristics (% contamination, callus, bud, root and oxidation), cytochemical test and microanalysis of X-rays evaluated. Concentrations of 0.5; 1.0 and 2.0 g L-1 of silicon did not differ in relation to callus formation. By the cytochemical test, all treatments presented embryogenic cells indicated by the reaction to acetic Carmine and, little reaction to the Evans blue associated with cell death. By the microanalysis of X-rays, the nutrients sulfur and phosphorus, presented significant absorption responses at the concentration of 1.5 g L-1 of silicon in comparison to the other concentrations. The silicon added to the culture medium has a response in the formation of cells with embryogenic potential in nodal segments of passion fruit. The concentration of 2.0 g L-1 of silicon acid (H4SiO4) has a high embryogenic potential in passionflower cells, Passiflora edulis f. Flavicarpa O. Deg.
机译：向培养基中添加硅可能有助于改善许多生长参数，包括胚胎发生和器官发生。这项研究的目的是评估在不同硅浓度下百香果（Passiflora edulis f。Flavicarpa O. Deg）在体外的胚发生潜能。在培养基中加入五种浓度的硅酸，分别接种植物的节段;浓度为0.0;浓度为0.0。 0.5; 1.0; 1.5和2.0 g L-1。 60天后，评估了视觉特征（污染百分比，愈伤组织，芽，根和氧化），细胞化学测试和X射线显微分析。浓度为0.5; 1.0和2.0 g L-1的硅在愈伤组织形成方面没有差异。通过细胞化学测试，所有处理均呈现出与乙酸胭脂红反应所指示的胚发生细胞，而与伊万斯蓝反应几乎没有与细胞死亡有关的反应。通过X射线的显微分析，与其他浓度相比，养分硫和磷在1.5 g L-1的硅浓度下表现出显着的吸收响应。添加到培养基中的硅在百香果的节段中具有胚发生潜能的细胞形成中具有响应。浓度为2.0 g L-1的硅酸（H4SiO4）在西番莲细胞西番莲中具有很高的胚发生潜能。 Flavicarpa O. Deg。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号