...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Medical Biochemistry >The Activity of Proximal Tubule Enzymes in the Urine of Cephalexin-Treated Patients
【24h】

The Activity of Proximal Tubule Enzymes in the Urine of Cephalexin-Treated Patients

机译：头孢氨苄治疗的患者尿液中近端小管酶的活性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Activity of Proximal Tubule Enzymes in the Urine of Cephalexin-Treated PatientsThe activities of alanine aminopeptidase (AAP), γ-glutamyltransferase (GGT) and N-acetyl-β-D-glucosaminidase (NAG), enzymes dominantly localised in the epithelial proximal tubule cells, were measured with an aim of determining the nephrotoxicity of a cephalosporin antibiotic cephalexin. Enzymatic activities were measured in the 12-h urine samples of patients receiving cephalexin orally for 15 days in daily doses of 50 mg/kg body mass against Gram-positive infections of the respiratory or urinary tract. The same enzymes were determined in the 12-h urine samples of the corresponding control. Both the control and the experimental group consisted of 30 examinees of both sexes, age range 3-10 years. Statistically significant differences in AAP and GGT activities expressed as U/mmol creatinine were recorded after 12 days of cephalexin therapy in comparison with the control (p < 0.01). At the same time, no significant differences in NAG activity of the patients in relation to the control were observed during the entire course of the therapy. Based on the obtained results it can be concluded that treatment of 3-10 years old patients with the applied cephalexin doses for 15 days results in mild nephrotoxic changes close to the end of therapy accompanied by increased activities of AAP and GGT, the enzymes known as very sensitive indicators of nephrotoxicity. The results showing that during the entire period of cephalexin application no changes in NAG, as a lysosomal enzyme, were observed, could be taken as a proof that this antibiotic did not lead to severe injuries of epithelial proximal tubule cells at the level of cell organelles.
机译：头孢氨苄治疗的患者尿液中近端小管酶的活性丙氨酸氨基肽酶（AAP），γ-谷氨酰转移酶（GGT）和N-乙酰基-β-D-氨基葡萄糖苷酶（NAG）的活性主要位于上皮近端小管中为了确定头孢菌素抗生素头孢氨苄的肾毒性，对细胞进行了测定。在每天口服剂量为50 mg / kg体重的头孢氨苄15天的患者的12小时尿液样本中，测量其酶活性对呼吸道或泌尿道的革兰氏阳性感染。在相应对照的12小时尿液样品中测定了相同的酶。对照组和实验组均由30名男女受试者组成，年龄范围为3-10岁。在头孢氨苄治疗12天后，与对照组相比，记录了以U / mmol肌酐表示的AAP和GGT活性的统计学差异（p <0.01）。同时，在整个治疗过程中，与对照组相比，患者的NAG活性均无明显差异。根据获得的结果，可以得出结论，用3到10岁的头孢氨苄剂量治疗15天会在治疗结束时导致轻度的肾毒性改变，同时伴随着AAP和GGT的活性增加，这种酶称为肾毒性非常敏感的指标。结果表明，在头孢氨苄的整个使用过程中，未观察到作为溶酶体酶的NAG的变化，可以作为该抗生素不会在细胞器水平上导致上皮近端小管细胞严重损伤的证据。 。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号