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首页> 外文期刊>Journal of medical bacteriology. >The Study of Antimicrobial Activities of Partially Purified Cyclotide Content and Crude Extracts from Viola tricolor
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The Study of Antimicrobial Activities of Partially Purified Cyclotide Content and Crude Extracts from Viola tricolor

机译：三色中提琴中部分纯化的环氧化物和粗提物的抗菌活性研究

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Background: Searching for the potent natural antibiotics will improve the treatment of most infectious diseases. Cyclotides are potent active peptides derived from some plant families like Violaceae. Viola tricolor (the pansy) has been found to have numerous cyclotides. Nowadays cyclotides attract more attention for their antibacterial activities. The current research studied the antimicrobial properties of semi-purified cyclotides from V. tricolor.?Methods: Extraction and purification of cyclotides from Iranian Viola tricolor were performed by fractionation and solid phase extraction methods, and their antimicrobial effects were studied against several bacterial strains using diffusion assays. Also, attendance of cyclotide in the extract was verified by Tricine-SDS page and spectroscopic methods.?Results: Antimicrobial effects of semi-purified cyclotides and crude extracts resulted in the antibacterial activity potential of V. tricolor totally extracted samples against gram negative bacteria, E.coli and P. aeruginosa. However, there is need for optimizing the assay method and the culture media.?Conclusion: Viola tricolor as a remedy represents the antibacterial potential, which may not be unrelated to its cyclotide content although the effectiveness of cyclotides may also differ because of their synergism, natural structure and bioactivities, the amount of purified content, and the way they were assayed.
机译：背景：寻找有效的天然抗生素将改善大多数传染病的治疗。环肽是衍生自某些植物科如堇菜科的有效活性肽。已发现三色中提琴（三色堇）具有许多环氧化物。如今，环磷酰胺因其抗菌活性而受到更多关注。方法：采用分馏和固相萃取的方法从伊朗三色堇菜中提取和纯化环磷酰胺，并研究其对几种细菌的抑菌作用。扩散测定。此外，通过Tricine-SDS页和光谱方法验证了提取物中环糊精的存在。结果：半纯化的环糊精和粗提取物的抗菌作用导致了三色青色全提取样品对革兰氏阴性细菌的抗菌活性，大肠杆菌和铜绿假单胞菌。但是，有必要优化测定方法和培养基。结论：三色堇菜是一种代表抗菌潜能的药物，尽管由于其协同作用，其有效性可能也有所不同，但它的环氧化物含量可能与其无关。天然结构和生物活性，纯化成分的量以及它们的测定方法。

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