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首页> 外文期刊>Journal of medical bacteriology. >Prevalence of IS256 among Ica-Positive and Biofilm Non-Producing Staphylococcus epidermidis Clinical Isolates
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Prevalence of IS256 among Ica-Positive and Biofilm Non-Producing Staphylococcus epidermidis Clinical Isolates

机译：Ica阳性和生物膜非生产性表皮葡萄球菌临床分离株中IS256的患病率

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Background: S. epidermidis is one of predominant members of human normal microflora, however it may be the main cause of nosocomial infections related to medical devices put into the body and thus the biofilm formation is a main route for pathogenesis which is affected by icaADBC operon. In this study, the prevalence of IS256 sequence among ica-positive and biofilm non-producer clinical isolates of S. epidermidis was investigated.?Methods: In this study, 100 clinical isolates of S. epidermidis were collected from different infections. The IS256 sequence, icaADBC operon and biofilm formation by microtiter plate assay were evaluated among them. The antibiotic susceptibility of these isolates was done with disc diffusion by using cefoxitin, ciprofloxacin, erythromycin, gentamycin, oxacillin and tetracycline discs.?Results: Of 100 isolates, 18 (18%) were ica operon-positive from which 18%, 14%, 16% and 17% contained icaA, icaD, icaB and icaC genes, respectively. Moreover, 14 of 18 (77.77%) ica-positive isolates amplified the IS256 gene. The biofilm formation by microtiter plate assay showed that 18 (18%) isolates were strong biofilm producers, 21 (21%) produced intermediate level biofilm and 14 (14%) and 47 (47%) isolates were weak and non-biofilm producers, respectively. in the antibiotic susceptibility test, the majority of isolates were resistant to oxacillin and lowest resistance was against ciprofloxacin.Conclusion: The statistical analysis with p<0.05 exhibited that there was a reverse relation between biofilm production and the insertion of IS256, and in fact the higher prevalence of IS256 among isolates, the biofilm formation declined. Data showed that amongst most of ica-positive isolates, the IS256 was detected and therefore other genetic factors affect the expression of this operon.
机译：背景：表皮葡萄球菌是人类正常菌群的主要成员之一，但它可能是与体内医疗器械相关的医院感染的主要原因，因此生物膜的形成是受icaADBC操纵子影响的发病机理的主要途径。在这项研究中，调查了表皮葡萄球菌的ica阳性和生物膜非生产者临床分离株中IS256序列的普遍性。方法：在这项研究中，从不同的感染中收集了100株临床表皮葡萄球菌。在其中评估了IS256序列，icaADBC操纵子和通过微量滴定板测定的生物膜形成。通过使用头孢西丁，环丙沙星，红霉素，庆大霉素，奥沙西林和四环素盘片对这些分离株进行药敏试验。结果：在100个分离株中，ica操纵子阳性的有18个（占18％），其中18％，14％为ica操纵子阳性。分别有16％和17％包含icaA，icaD，icaB和icaC基因。此外，在18株ica阳性分离物中，有14株（77.77％）扩增了IS256基因。通过微量滴定板分析的生物膜形成表明，有18（18％）的分离物是强生物膜生产者，有21（21％）的是中等水平的生物膜生产者，有14（14％）和47（47％）的分离物是弱的和非生物膜的生产者，分别。在抗生素敏感性试验中，大多数分离株对奥沙西林耐药，对环丙沙星的耐药性最低。结论：p <0.05的统计分析表明，生物膜产量与IS256的插入存在反比关系，实际上分离株中IS256的患病率较高，生物膜形成下降。数据显示，在大多数ica阳性分离株中，检测到IS256，因此其他遗传因素影响该操纵子的表达。

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