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首页> 外文期刊>Journal of Microbiology, Biotechnology and Food Sciences >IN VITRO ASSESSMENT OF OCTYLPHENOL IMPACT ON SPERMATOZOA MOTILE PARAMETERS
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IN VITRO ASSESSMENT OF OCTYLPHENOL IMPACT ON SPERMATOZOA MOTILE PARAMETERS

机译：邻苯二酚对精子活动参数的影响的体外评估

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In this study we examined the effect of various concentrations of octylphenol (OP; Sigma-Aldrich, St. Louis, USA) (1, 10, 100 and 200 μg/mL) dissolved in 1% ethanol (Sigma-Aldrich, Bratislava, Slovak Republic) on the motile parameters of bovine spermatozoa during cultivation times 0 h, 2 h, 4 h and 6 h. The control spermatozoa (Ctrl) group was cultured with physiological saline solution (sodium chloride 0.9% w/v, Bieffe Medital, Italy). The motility analysis was carried out using a CASA (Computer Assisted Semen Analyzer) system – SpermVisionTM program (MiniT?b, Tiefenbach, Germany) with the Olympus BX 51 microscope (Olympus, Tokyo, Japan). The following parameters were evaluated: percentage of motile spermatozoa (motility > 5 μm/s), percentage of progressive motile spermatozoa (motility > 20 μm/s), distance average path (DAP; μm) and velocity average path (VAP; μm/s). Data were statistically analyzed using program GraphPad Prism 3.02 (GraphPad Software Incorporated, San Diego, California, USA). One-way analysis of variance (ANOVA) and the Dunnett′s multiple comparison test were used for statistical evaluations. The control group (medium without OP) was compared to the experimental groups (exposed to different concentrations of OP). Significant differences (P<0.001 and P<0.05) between the control group and experimental groups were recorded. Obtained data from our study showed a decreased spermatozoa motility and progressive motility in all experimental groups with the addition of OP in comparison to the control group during all time periods. The evaluation of OP influence on the motile parameters DAP and VAP indicated a significant decrease and increase in experimental groups with 1, 10 and 200 μg/mL of OP. In conclusion, our results confirm that octylphenol have the detrimental effect on the rated parameters of spermatozoa motility during short-term cultivation.
机译：在这项研究中，我们研究了溶解在1％乙醇（Sigma-Aldrich，布拉迪斯拉发，斯洛伐克）中的各种浓度的辛基酚（OP; Sigma-Aldrich，美国圣路易斯）的影响（1、10、100和200μg/ mL）。牛精子在培养时间0 h，2 h，4 h和6 h时的运动参数。对照组的精子（Ctrl）组用生理盐水溶液（0.9％w / v的氯化钠，意大利Bieffe Medital）培养。使用CASA（计算机辅助精液分析仪）系统– SpermVisionTM程序（MiniT？b，德国蒂芬巴赫，德国）和Olympus BX 51显微镜（Olympus，东京，日本）进行运动分析。评估了以下参数：活动精子的百分比（运动> 5μm/ s），进行性活动精子的百分比（运动> 20μm/ s），距离平均路径（DAP;μm）和速度平均路径（VAP;μm/ s）。使用程序GraphPad Prism 3.02（GraphPad Software Incorporated，圣地亚哥，加利福尼亚，美国）对数据进行统计分析。使用单向方差分析（ANOVA）和Dunnett的多重比较检验进行统计评估。将对照组（不含OP的培养基）与实验组（暴露于不同浓度的OP）进行比较。记录对照组和实验组之间的显着差异（P <0.001和P <0.05）。从我们的研究中获得的数据显示，与对照组相比，在所有时间段内，所有实验组的精子活动性和进行性活动性均下降，并且添加了OP。对OP对运动参数DAP和VAP的影响的评估表明，实验组分别以1、10和200μg/ mL的OP显着减少和增加。总之，我们的结果证实辛基苯酚对短期培养过程中的精子活力指标有不利影响。

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