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首页> 外文期刊>Journal of Microbiology, Biotechnology and Food Sciences >MC4R AND PGAM2 GENES POLYMORPHISM ASSOCIATION WITH PRODUCTION TRAITS IN RABBIT (ORYCTOLAGUS CUNICULUS)
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MC4R AND PGAM2 GENES POLYMORPHISM ASSOCIATION WITH PRODUCTION TRAITS IN RABBIT (ORYCTOLAGUS CUNICULUS)

机译：兔MC4R和PGAM2基因多态性与生产性状的关系

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Mutations of the melanocortin-4 receptor (MC4R) gene are associated with body weight, obesity, and growth. PGAM2 is associated with drip loss, meat colour, fat deposition, lean content, muscle fiber diameter and carcass traits. The present study had been carried out to investigate the association between a single nucleotide polymorphism (SNP) of MC4R (101G>A) and PGAM2 (195C>T) gene and selected production traits in rabbits. Genomic DNA for all analyses was extracted from muscle (biceps femoris) using NucleoSpin kit. The c.101G>A (MC4R) and c.195C>T (PGAM2) was genotyped by PCR-RFLP in a total of 44 rabbits. The average allele frequency was 0.63 for allele A and 0.37 for G, resp. 0.66 for allele C and 0.34 for T. The association results revealed the AA genotype of c.101G>A was associated significantly (P < 0.05) with greater live body weight. Statistically significant association of the genotypes with other production traits was not observed.
机译：melanocortin-4受体（MC4R）基因的突变与体重，肥胖和生长有关。 PGAM2与流失，肉色，脂肪沉积，瘦肉含量，肌纤维直径和car体性状有关。本研究旨在研究家兔MC4R（101G> A）和PGAM2（195C> T）基因的单核苷酸多态性（SNP）与所选生产性状的关系。使用NucleoSpin试剂盒从肌肉（股二头肌）中提取所有分析的基因组DNA。通过PCR-RFLP对44只家兔进行c.101G> A（MC4R）和c.195C> T（PGAM2）基因分型。平均等位基因频率分别为A等位基因0.63，G等位基因0.37。等位基因C为0.66，T基因为0.34。关联结果显示，c.101G> A的AA基因型与更大的活体重显着相关（P <0.05）。没有观察到基因型与其他生产性状的统计学显着关联。

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