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首页> 外文期刊>Journal of Molecular Biology Research >Hydroxyl Radical (oOH) Scavenger Power of Tris (hydroxymethyl) Compared to Phosphate Buffer
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Hydroxyl Radical (oOH) Scavenger Power of Tris (hydroxymethyl) Compared to Phosphate Buffer

机译：Tris（羟甲基）与磷酸缓冲液相比的羟基自由基（oOH）清除剂能力

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摘要

Tris and phosphate buffer are regularly used in experimental investigations. These buffers might have radical scavenger properties toward different kinds of Reactive Oxygen Species (ROS) produced in solutions during chemical reactions like Fenton reaction and gamma radiolysis. Hydroxyl radicals (oOH) are the most reactive and oxidizing agents having a great potential in oxidization of macromolecules like DNA and proteins. This in vitro study was aimed to evaluate radio-protective effects of Tris and phosphate buffer toward hydroxyl radicals generated by Fenton reaction. Hence, oOH radicals were produced using a mixture of Hydrogen Peroxideand Ferrous Sulfate, called Fenton system. Human serum albumin (500μM) was prepared in Tris (10mM) and phosphate buffer (10mM), separately. These two samples were incubated with Fenton reaction (Ferrous Sulfate + Hydrogen peroxide) (10 mM) for 30 minutes and carbonyl groups were quantified by spectrophotometric carbonylation assay. The results of this study revealed the values of 1.04 ± 0.02 and 1.73 ± 0.03 for Tris and phosphate buffer treated samples, respectively. In conclusion, these findings confirmed that Tris buffer is a stronger radical scavenger toward oOH radicals than phosphate buffer.
机译：实验研究中经常使用Tris和磷酸盐缓冲液。这些缓冲液可能对Fenton反应和伽马射线分解等化学反应期间溶液中产生的不同种类的活性氧（ROS）具有自由基清除剂性能。羟基自由基（oOH）是最具反应性的氧化剂，在诸如DNA和蛋白质的大分子氧化中具有很大的潜力。这项体外研究旨在评估Tris和磷酸盐缓冲液对Fenton反应产生的羟基自由基的放射防护作用。因此，使用过氧化氢和硫酸亚铁的混合物（称为Fenton系统）产生了oOH自由基。分别在Tris（10mM）和磷酸盐缓冲液（10mM）中制备人血清白蛋白（500μM）。将这两个样品与Fenton反应（硫酸亚铁+过氧化氢）（10 mM）温育30分钟，并通过分光光度羰基化测定法对羰基进行定量。这项研究的结果表明，Tris和磷酸盐缓冲液处理过的样品的值分别为1.04±0.02和1.73±0.03。总之，这些发现证实了Tris缓冲剂比磷酸缓冲剂对oOH自由基的清除能力更强。

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