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首页> 外文期刊>Journal of Molecular Biology Research >Hydroxyl Radical (oOH) Scavenger Power of Tris (hydroxymethyl) Compared to Phosphate Buffer
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Hydroxyl Radical (oOH) Scavenger Power of Tris (hydroxymethyl) Compared to Phosphate Buffer

机译：Tris（羟甲基）与磷酸缓冲液相比的羟基自由基（oOH）清除剂能力

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摘要

Tris and phosphate buffer are regularly used in experimental investigations.These buffers might have radical scavenger properties toward different kinds of Reactive Oxygen Species (ROS) produced in solutions during chemical reactions like Fenton reaction and gamma radiolysis.Hydroxyl radicals (oOH) are the most reactive and oxidizing agents having a great potential in oxidization of macromolecules like DNA and proteins.This in vitro study was aimed to evaluate radio-protective effects of Tris and phosphate buffer toward hydroxyl radicals generated by Fenton reaction.Hence, oOH radicals were produced using a mixture of Hydrogen Peroxideand Ferrous Sulfate, called Fenton system.Human serum albumin (500μM) was prepared in Tris (10mM) and phosphate buffer (10mM), separately.These two samples were incubated with Fenton reaction (Ferrous Sulfate + Hydrogen peroxide) (10 mM) for 30 minutes and carbonyl groups were quantified by spectrophotometric carbonylation assay.The results of this study revealed the values of 1.04 ± 0.02 and 1.73 ± 0.03 for Tris and phosphate buffer treated samples, respectively.In conclusion, these findings confirmed that Tris buffer is a stronger radical scavenger toward oOH radicals than phosphate buffer.
机译：实验研究中经常使用Tris和磷酸盐缓冲液，这些缓冲液可能对Fenton反应和伽马射线分解等化学反应过程中溶液中产生的不同种类的活性氧（ROS）具有自由基清除剂的特性。羟自由基（oOH）是最活泼的这项体外研究旨在评估Tris和磷酸盐缓冲液对Fenton反应产生的羟基自由基的放射防护作用。因此，使用混合物产生了oOH自由基分别用Tris（10mM）和磷酸盐缓冲液（10mM）制备人血清白蛋白（500μM），将这两个样品与Fenton反应（硫酸亚铁+过氧化氢）（10 mM）一起孵育。 ）30分钟，并通过分光光度羰基化测定法对羰基进行定量分析。分别揭示了Tris和磷酸盐缓冲液处理样品的1.04±0.02和1.73±0.03值。总而言之，这些发现证实了Tris缓冲液比磷酸缓冲液对oOH自由基的清除能力更强。

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