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首页> 外文期刊>Journal of Molecular Biology Research >Genetic Diversity of Rhizobia Nodulating Alfalfa in Iraq as a Source of More Efficient Drought Tolerance Strains
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Genetic Diversity of Rhizobia Nodulating Alfalfa in Iraq as a Source of More Efficient Drought Tolerance Strains

机译：根瘤菌根瘤紫花苜蓿的遗传多样性作为更有效的耐旱菌株的来源

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Sinorhizobium meliloti is a gram-negative, soil bacteria, which gain a huge importance deserved to their capability in fixing nitrogen symbiotically with an important fodder crop legume-alfalfa (Medicago sativa). This study aims to (i): isolate indigenous Sinorhizobium meliloti from different field sites in Iraq; (ii): assess the isolates tolerance to induced water shortage using polyethylene glycol-6000; (iii): evaluate genetic diversity and genetic relationships among isolates of natural population with drought tolerant abilities. Drought tolerance analysis disclose great variations between Sinorhizobium isolates, the highest tolerant isolates to drought were 12 from whole thirty (40%), endured from -3 up to -4 MPa(Mega pascal), at the same time the drought sensitive isolates tolerated up to -1.5 MPa, but for isolate Bs58 which tolerated up to -1 MPa. The growth decrease with the increase of drought stress. REP-PCR method was an excellent technique to distinguish between the S. isolates based on their tolerance to different water potential levels, the REP-PCR gave a significant band about 700bp (base pair) in size, which were present in all drought moderate and tolerant isolates, while this band was absent in all drought sensitive isolates(except one isolate). Also the ERIC-PCR method cleared the absence of two significant bands 300bp and 600bp from all drought sensitive isolates. UPGMA analysis based on rep-PCR revealed two groups branching at a similarity of 35%, first group included only one isolate Bs16, which was a drought moderate tolerant; second group contained the rest of isolates, yet the latter splits into two subgroups with 68% similarity; the first subgroup comprised all sensitive isolates with 80% similarity among them, the second subgroup included the tolerant and moderate isolates with similarity 88%.
机译：苜蓿中华根瘤菌是一种革兰氏阴性土壤细菌，由于其与重要的饲料作物豆科植物苜蓿（Medicago sativa）共生固氮的能力而变得极为重要。这项研究的目的是：（i）从伊拉克不同的野外场所中分离出本地的中华根瘤菌; （ii）：使用聚乙二醇-6000评估菌株对诱导缺水的耐受性; （iii）：评估具有耐旱能力的自然种群分离株之间的遗传多样性和遗传关系。耐旱性分析揭示了中华根瘤菌之间的差异很大，最高的耐旱菌株为全三十株中的十二株（占40％），耐-3至-4 MPa（兆帕斯卡），同时耐旱性最高的菌株被耐受到-1.5 MPa，但对于耐受高达-1 MPa的分离物Bs58。生长随干旱胁迫的增加而减少。 REP-PCR方法是根据分离株对不同水势水平的耐受性来区分的优良技术，REP-PCR产生了一条大约700bp（碱基对）的显着条带，该条带存在于所有干旱中等和中等干旱地区耐性菌株，而所有对干旱敏感的菌株（一个菌株除外）都没有该条带。此外，ERIC-PCR方法还清除了所有对干旱敏感的分离株的两个显着条带300bp和600bp。根据rep-PCR进行的UPGMA分析显示，两组的分支相似度为35％，第一组仅包含一个分离株Bs16，它具有耐旱性。第二组包含其余的分离株，但后者分为具有68％相似性的两个亚组。第一个亚组包括所有敏感性分离株，它们之间的相似度为80％，第二个亚组包括耐受性和中等分离株，相似性为88％。

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