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Simple Method to Extract DNA from Mammalian Whole Blood

机译:从哺乳动物全血中提取DNA的简单方法

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DNA isolation from cells is the 1st and the foremost step involved in molecular biology. The high quality and purity of extracted DNA and simplicity as well as low cost of the extraction method is an important consideration for a molecular biologist. Genomic mammalian DNA is very long and extraction must avoid high levels of centrifuge and severe shakes. There are several methods to extract DNA, including: Boiling, Salting out, Phenol-Chloroform, Isopropanol Precipitation methods. In this study, a method which seems to be superior to salting out and other methods has been developed. The extraction of DNA by this method is carried out from leukocytes. This modified method is simple, cheap and has a wider application than other methods.
机译:从细胞中分离DNA是分子生物学涉及的第一步也是最重要的一步。提取的DNA的高质量和纯度以及提取方法的简便性和低成本是分子生物学家的重要考虑因素。哺乳动物的基因组DNA很长,提取时必须避免高水平的离心和剧烈摇动。有几种提取DNA的方法,包括:沸腾,盐析,苯酚-氯仿,异丙醇沉淀法。在这项研究中,已经开发出一种似乎比盐析和其他方法更好的方法。通过这种方法从白细胞中提取DNA。这种改进的方法简单,便宜,并且比其他方法具有更广泛的应用。

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