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首页> 外文期刊>Journal of Molecular Genetics >Detection of Polymorphisms in the Bovine Leptin (LEP) Gene: Association of a Single Nucleotide Polymorphism With Breeding Value of Milk Traits in Iranian Holstein Cattle
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Detection of Polymorphisms in the Bovine Leptin (LEP) Gene: Association of a Single Nucleotide Polymorphism With Breeding Value of Milk Traits in Iranian Holstein Cattle

机译：牛瘦素（LEP）基因中的多态性的检测：单核苷酸多态性与伊朗荷斯坦牛的牛奶性状的育种价值的关联。

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The objective of this study was to assess the association of polymorphism in leptin gene with breeding value of milk traits in Iranian Holstein cattle. Traits analyzed were breeding values of Milk and Fat production. A strategy employing polymerase chain reaction was used to amplify a 422 bp from blood, semen. Digestion of polymerase chain reaction products with Sau3AI revealed two alleles: Allele A was 390, 32 fragment and allele B was 303, 88 and 32 (only 303 fragment visible on the gel). Three patterns were observed frequencies were 0.900, 0.100 and 0.00 for AA, AB and BB, respectively (p≤0.21). The AB bulls were superior for milk fat production, in relation to homozygous AA. The Bovine leptin gene is strong candidates in the dairy cattle QTL search. This polymorphism could be further evaluated for marker assisted selection and the developed PCR methodology would expedite screening for large numbers of animal required for such studies.
机译：这项研究的目的是评估瘦素基因多态性与伊朗荷斯坦奶牛奶特性育种价值的关联。分析的性状是牛奶和脂肪产量的育种价值。使用聚合酶链反应的策略被用于从血液精液中扩增422bp。用Sau3AI消化聚合酶链反应产物可发现两个等位基因：等位基因A为390、32个片段，等位基因B为303、88和32（在凝胶上仅可见303片段）。观察到三种模式，AA，AB和BB的频率分别为0.900、0.100和0.00（p≤0.21）。与纯合AA相比，AB公牛的乳脂产量更高。牛瘦素基因是奶牛QTL搜索的有力候选者。可以进一步评估这种多态性以进行标记辅助选择，而发达的PCR方法将加快筛选此类研究所需的大量动物的速度。

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