Lack of Correlation Between HERV-K Expression and HIV-1 Viral Load in Plasma Specimens

Daniel Esqueda; Fangling Xu; Yuliana Moore; Zhen Yang; Gengming Huang; Patrick A. Lennon; Peter C. Hu; Jianli Dong

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Lack of Correlation Between HERV-K Expression and HIV-1 Viral Load in Plasma Specimens

机译：血浆样本中HERV-K表达与HIV-1病毒载量之间缺乏相关性

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HERV-K viral RNA has been reported in plasma specimens of HIV-1 infected individuals. Emerging data support the regulation and functional interaction between HERV-K and HIV-1 which warrant development of accurate HERV-K assays to evaluate HERV-K activation. In this study, researchers examined HERV-K RNA expression after careful removal of contaminating cellular DNA using DNase I. Researchers found that DNase I digestion effectively reduced HERV-K RT-PCR positive signal. Researchers also found that levels of HERV-K expression did not correlate with HIV-1 viral load. The study is in agreement with the published studies on HERV-K activation in HIV-1 viral positive plasma specimens and in addition calls for careful removal of cellular DNA to accurately evaluate HERV-K RNA expression.

机译：在HIV-1感染者的血浆样本中已报道了HERV-K病毒RNA。新兴数据支持HERV-K和HIV-1之间的调节和功能相互作用，这保证了开发准确的HERV-K分析法以评估HERV-K活化的可能性。在这项研究中，研究人员在使用DNase I小心去除污染的细胞DNA后检查了HERV-K RNA的表达。研究人员发现，DNase I消化可有效降低HERV-K RT-PCR阳性信号。研究人员还发现，HERV-K表达水平与HIV-1病毒载量无关。该研究与已发表的关于HIV-1病毒阳性血浆标本中HERV-K活化的研究相一致，此外，还要求仔细去除细胞DNA以准确评估HERV-K RNA的表达。

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《Journal of Molecular Genetics》 |2011年第3期|共4页
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Daniel Esqueda; Fangling Xu; Yuliana Moore; Zhen Yang; Gengming Huang; Patrick A. Lennon; Peter C. Hu; Jianli Dong;
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1. Lack of Correlation between Seminal and Plasma HIV-1 Viral Loads is Associated with CD4 T Cell Depletion in Therapy-naïve HIV-1 + Patients [J] . Lauro FS Pinto-Neto, Nilo FR Vieira, Moacir Soprani, Memorias do Instituto Oswaldo Cruz . 2002,第4期

机译：未经治疗的HIV-1 +患者中精浆和血浆HIV-1病毒载量之间缺乏相关性与CD4 T细胞耗竭有关
2. Lack of Correlation between Seminal and Plasma HIV-1 Viral Loads is Associated with CD4T Cell Depletion in Therapy-na?ve HIV-1+ Patients [J] . Carla B Cunha, Lauro Fs Pinto-neto, Moacir Soprani, Memórias do Instituto Oswaldo Cruz . 2002,第4期

机译：初治HIV-1 +患者精浆和血浆HIV-1病毒载量之间缺乏相关性与CD4T细胞耗竭相关。
3. A new Real-Time-RT-PCR for quantitation of human endogenous retroviruses type K (HERV-K) RNA load in plasma samples: increased HERV-K RNA titers in HIV-1 patients with HAART non-suppressive regimens. [J] . Contreras Galindo R, Gonzalez M, Almodovar Camacho S, Journal of Virological Methods . 2006,第1a2期

机译：一种用于定量血浆样品中人类内源性K型逆转录病毒（HERV-K）RNA载量的新型实时RT-PCR：使用HAART非抑制方案的HIV-1患者中HERV-K RNA滴度增加。
4. SVM-enabled prognostic method for clinical decision making: The use of CD4 T-cell level and HIV-1 viral load for guiding treatment initiation and alteration [C] . Kasmiran Khairul A, Zomaya Albert Y, Mazari Ali A, 23rd IEEE International Symposium on Computer-Based Medical Systems . 2010

机译：支持SVM的临床决策预后方法：使用CD4 T细胞水平和HIV-1病毒载量指导治疗的开始和改变
5. Investigation of HERV-K (HML-2) expression during HIV-1 infection. [D] . Bhardwaj, Neeru. 2015

机译：调查HIV-1感染期间的HERV-K（HML-2）表达。
6. Evaluation of Effect of Specimen-Handling Parameters for Plasma Preparation Tubes on Viral Load Measurements Obtained by Using the Abbott RealTime HIV-1 Load Assay [O] . Helen Fernandes, Svetlana Morosyuk, Klara Abravaya, 2010

机译：通过雅培实时HIV-1负荷分析获得的血浆制备管样品处理参数对病毒负荷测量的影响评估
7. Lack of Correlation between Seminal and Plasma HIV-1 Viral Loads is Associated with CD4T Cell Depletion in Therapy-naïve HIV-1+ Patients [O] . Lauro FS Pinto-Neto, Nilo FR Vieira, Moacir Soprani, 2002

机译：精液和血浆中缺乏相关性HIV-1病毒载量与未接受治疗的HIV-1 +患者的CD4T细胞耗竭有关

Lack of Correlation Between HERV-K Expression and HIV-1 Viral Load in Plasma Specimens

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