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Isolation, Similarity and Subcellular Localisation of Transaldolase from Sugarcane (Saccharum officinarum)

机译:甘蔗糖蔗糖醛缩醛酶的分离,相似性和亚细胞定位

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This study focused on isolation, cloning of TAL from sugarcane. Transaldolase is one of the enzymes of the Pentose Phosphate Pathway (PPP). Transaldolase in non-oxidative phase of OPPP transfer a three carbon dihydroxyacetone moiety from sedoheptulose-7-phosphate and glyceraldehyde-3-phophate to produce Erythrose-4-Phophate (E4P) and fructose-6-phophate. E4P is the precursor for many secondary metabolic pathways including aromatic amino acids, lignin and flavonoid synthesis. Earlier studies revealed that OPPP is incomplete in the cytosol of plants as no genes encoding for a cytosolic TAL. Moreover, there is no study about the TAL genes from sugarcane until to date. Thus, the objective of this study is to isolate TAL gene from sugarcane, to compare its similarity with other plants, to determine its subcellular localization. A total of 1601 bp of TAL has been isolated by PCR. Similarity, studies by ClustalW revealed that TAL show highest similarity (75%) with Zea mays. Analysis of subcellular localization by using Target 1.1 revealed that of TAL from sugarcane was not located in the plastidic.
机译:这项研究的重点是从甘蔗中分离,克隆TAL。 Transaldolase是戊糖磷酸途径(PPP)的酶之一。在OPPP的非氧化相中的转醛缩酶将七碳七磷酸和3磷酸甘油醛中的三个碳二羟基丙酮部分转移,生成4磷酸赤藓酯(E4P)和6磷酸果糖。 E4P是许多次级代谢途径的前体,包括芳香族氨基酸,木质素和类黄酮合成。较早的研究表明,由于没有编码胞质TAL的基因,因此OPPP在植物的细胞质中并不完整。此外,迄今为止,还没有关于甘蔗中TAL基因的研究。因此,本研究的目的是从甘蔗中分离TAL基因,比较其与其他植物的相似性,以确定其亚细胞定位。通过PCR分离出总共1601bp的TAL。相似性,ClustalW的研究表明,TAL与玉米粥的相似性最高(75%)。通过使用目标1.1的亚细胞定位分析表明,来自甘蔗的TAL不在质体中。

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