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首页> 外文期刊>Journal of Molecular Genetics >Variants of the Diacylglycerol acyltransferase 1 (Dgat1) Gene in Sudanese Dairy Cattle (Kenana and Butana)
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Variants of the Diacylglycerol acyltransferase 1 (Dgat1) Gene in Sudanese Dairy Cattle (Kenana and Butana)

机译：苏丹奶牛（肯尼亚和Butana）中的二酰基甘油酰基转移酶1（Dgat1）基因的变体

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The aim of the study was the characterization of DGAT1 variants in Sudanese dairy cattle breeds. In this study, we examined 94 Kenana and 91 Butana dairy cattle from two regions of Sudan. We genotyped the DGAT1 sequence variant AJ318490.1:g.10433/10434 AA>GC that leads to the Lysine-Alanine substitution at position 232 (K232A) in the protein and the VNTR polymorphism in the promoter region. Genotyping was performed by allele specific PCR and PCR fragment lengths determination, respectively. In both breeds, the DGAT1 Lysine variant (232K) that is associated with high fat and protein content as well as high fat yield in other breeds is the high frequent allele. The frequencies of the 232K allele were 96.3 and 84.6% in Kenana and Butana breeds, respectively. At the DGAT1 promoter VNTR locus, four alleles containing four to seven repeats of the 18 bp motif were found in both breeds. The highest frequent allele was the VNTR allele 3 containing five repeats with 60.4 and 57.5% in Kenana and Butana breeds, respectively. In conclusion, the two examined Sudanese dairy cattle breeds do not differ in allele frequencies at the DGAT1 locus.
机译：该研究的目的是鉴定苏丹奶牛品种中DGAT1的变异。在这项研究中，我们检查了来自苏丹两个地区的94头Kenana和91头Butana奶牛。我们对DGAT1序列变体AJ318490.1：g.10433 / 10434 AA> GC进行基因分型，该变体导致蛋白质中232位（K232A）处的赖氨酸-丙氨酸取代和启动子区域中的VNTR多态性。通过等位基因特异性PCR和PCR片段长度测定分别进行基因分型。在两个品种中，DGAT1赖氨酸变体（232K）与高脂肪和高蛋白含量以及其他品种中的高脂肪产量相关，是等位基因频率很高。 Kenana和Butana品种的232K等位基因频率分别为96.3和84.6％。在DGAT1启动子VNTR位点，在两个品种中发现了四个等位基因，这些等位基因包含18个碱基对基序的4至7个重复。频率最高的等位基因是VNTR等位基因3，在Kenana和Butana品种中分别含有5个重复序列，分别为60.4和57.5％。总之，两个受检查的苏丹奶牛品种在DGAT1位点的等位基因频率没有差异。

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