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首页> 外文期刊>Journal of Oral Microbiology >Comparison of paperpoint and curette sampling of subgingival microbiome composition as analyzed by 16S rRNA gene amplicon sequencing
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Comparison of paperpoint and curette sampling of subgingival microbiome composition as analyzed by 16S rRNA gene amplicon sequencing

机译：通过16S rRNA基因扩增子测序分析龈下微生物组组成的纸样和刮匙采样的比较

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Objective: Our aim was to compare the subgingival microbiome composition in samples collected by curettes and paperpoints.Methods: Subgingival plaque of rheumatoid arthritis patients with periodontitis (N=66) or gingivitis (N=15) was collected at two timepoints by sterile curettes and paperpoints. Unused sterile paperpoints were included as controls. The microbial DNA was processed for 16S rRNA gene amplicon sequencing. The data was processed and clustered into Operational Taxonomic Units (OTUs) and assessed by multivariate analyses.Results: Unused paperpoints showed various levels of DNA contamination. The OTUs specific for unused paperpoints were classified as Exiguobacterium, Enterococcus, Methylobacterium, Aquabacterium and Pseudomonas and were removed from the dataset.Microbial profiles of curette samples differed significantly from the paperpoint samples (PERMANOVA, p=0.0009, F=3.167). The paperpoint samples had significantly higher proportion of OTUs classified as Streptococcus, Gemella, Pa...
机译：目的：我们的目的是比较刮匙和纸点收集的样本中龈下微生物组的组成。方法：在两个时间点通过无菌刮匙收集类风湿性关节炎牙周炎（N = 66）或牙龈炎（N = 15）患者的龈下菌斑。论文要点。包括未使用的无菌试纸作为对照。将微生物DNA进行16S rRNA基因扩增子测序。数据经过处理并聚类为操作分类单位（OTUs），并通过多变量分析进行了评估。结果：未使用的论点显示出不同水平的DNA污染。针对未使用的纸质点的OTU分类为Exiguobacterium，肠球菌，甲基杆菌，水杆菌和假单胞菌，并从数据集中删除。刮匙样品的微生物特征与纸质点显着不同（PERMANOVA，p = 0.0009，F = 3.167）。纸质样本的OTU比例显着较高，被分类为链球菌，双歧杆菌，帕氏菌...

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