...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Nutrition & Food Sciences >Analysis of Microbial Populations and Metabolism of Anthocyanins by Mice Gut Microflora Fed with Blackberry Powder
【24h】

Analysis of Microbial Populations and Metabolism of Anthocyanins by Mice Gut Microflora Fed with Blackberry Powder

机译：黑莓粉喂养的小鼠肠道菌群对微生物种群和花色苷代谢的分析

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Blackberries contain non-nutritive phytochemicals which are abundant in berry fruits. Among these chemicals, anthocyanins are responsible for the bright colors seen in many fruits and plant as well as have potential health properties against chronic diseases like cancer, cardiovascular disease, diabetes, and obesity. Therefore, two anthocyanins, cyanidin-3-glucoside and cyanidin-3-rutinoside were adopted to evaluate metabolism rate by cecal microflora which were acquired from four treatments 1) lean control (L-CTL), 2) obese control (O-CTL), 3) aged blackberries supplemented at 10% (w/w) (O-AB10), and 4) fresh blackberries supplemented at 10% (w/w) (OFB10). Furthermore, a non-culture based technique; polymerase chain reaction-based denaturing gradient gel electrophoresis (PCR-based DGGE) and further sequencing were performed to identify microbial populations. There were differences in the metabolism rates of two anthocyanins (cyanidin-3-glucoside and cyanidin-3-rutinoside) between two control groups and two treatment groups while no differences were shown within two control groups. The sequencing results based on DGGE represented that Lachnospiraceae bacteria found predominantly in blackberry treatment mice, Lactobacillus johnsonii was dominant species in both control and treatment groups. Bifodobacteriumpseudolongum was identified in only obese control samples. In conclusion, differences in diets and body phenotype can have an effect on gut microflora and subsequently affect the metabolism rate of anthocyanins. Also, blackberry treatment groups demonstrated a more rapid anthocyanin degradation rate than the un-adapted control groups.
机译：黑莓中含有丰富的非营养性植物化学物质。在这些化学物质中，花色苷是造成许多水果和植物中鲜艳颜色的原因，并且具有潜在的健康特性，可抵抗癌症，心血管疾病，糖尿病和肥胖症等慢性疾病。因此，采用两种花色苷花色苷-3-葡糖苷和花色苷-3-芸香苷来评估盲肠菌群的代谢率，这些盲肠菌群是从以下四种处理中获得的：1）瘦肉对照（L-CTL），2）肥胖对照（O-CTL） ，3）以10％（w / w）（O-AB10）补充的老黑莓，和4）以10％（w / w）（OFB10）补充的新鲜黑莓。此外，基于非文化的技术;进行基于聚合酶链反应的变性梯度凝胶电泳（基于PCR的DGGE）和进一步测序，以鉴定微生物种群。在两个对照组和两个治疗组之间，两种花色苷（花青素-3-葡萄糖苷和花青素-3-芸苔苷）的代谢率存在差异，而在两个对照组中则没有差异。基于DGGE的测序结果表明，在黑莓治疗小鼠中主要发现了乳酸杆菌科细菌，约翰逊乳杆菌在对照组和治疗组中都是优势种。仅在肥胖对照样品中鉴定出假双歧杆菌。总之，饮食和身体表型的差异会影响肠道菌群，进而影响花青素的代谢率。而且，黑莓治疗组比未适应的对照组显示出更快的花色苷降解速率。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号