The E. coli single protein production system for production and structural analysis of membrane proteins

Lili Mao; S. Thangminlal Vaiphei; Tsutomu Shimazu; William M. Schneider; Yuefeng Tang; Rajeswari Mani; Monica J. Roth; Gaetano T. Montelione; Masayori Inouye

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The E. coli single protein production system for production and structural analysis of membrane proteins

机译：用于膜蛋白生产和结构分析的大肠杆菌单蛋白生产系统

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At present, only 0.9% of PDB-deposited structures are of membrane proteins in spite of the fact that membrane proteins constitute approximately 30% of total proteins in most genomes from bacteria to humans. Here we address some of the major bottlenecks in the structural studies of membrane proteins and discuss the ability of the new technology, the Single-Protein Production system, to help solve these bottlenecks.

机译：目前，尽管从细菌到人类的大多数基因组中，膜蛋白占总蛋白的约30％，但只有0.9％的PDB沉积结构是膜蛋白。在这里，我们解决了膜蛋白结构研究中的一些主要瓶颈，并讨论了新技术“单蛋白生产系统”帮助解决这些瓶颈的能力。

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《Journal of structural and functional genomics》 |2010年第1期|共4页
作者
Lili Mao; S. Thangminlal Vaiphei; Tsutomu Shimazu; William M. Schneider; Yuefeng Tang; Rajeswari Mani; Monica J. Roth; Gaetano T. Montelione; Masayori Inouye;
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1. The Use of the Condensed Single Protein Production System for Isotope-Labeled Outer Membrane Proteins, OmpA and OmpX in E. coli [J] . S. Thangminlal Vaiphei, Yuefeng Tang, Gaetano T. Montelione, Molecular Biotechnology . 2011,第3期

机译：浓缩单蛋白生产系统用于大肠杆菌中同位素标记的外膜蛋白，OmpA和OmpX的使用
2. The use of the condensed single protein production system for isotope-labeled outer membrane proteins, OmpA and OmpX in E. coli [J] . Vaiphei S.T., Tang Y., Montelione G.T., Molecular biotechnology . 2011,第3期

机译：浓缩的单一蛋白质生产系统用于大肠杆菌中同位素标记的外膜蛋白质OmpA和OmpX的使用
3. Production of Recombinant Mouse Flower Protein in E. coli: Application of Mistic Fusion to Improving the Expression of Membrane Proteins [J] . M. Marenchino, R. Gonzalez, J. Lopez-Alonso, Journal of biomolecular techniques :JBT. . 2011,第Suppl期

机译：重组小鼠花蛋白在大肠杆菌中的生产：Mistic融合蛋白在改善膜蛋白表达中的应用
4. MODEL-BASED ADAPTIVE CONTROL OF ACETATE CONCENTRATION DURING THE PRODUCTION OF RECOMBINANT PROTEINS WITH E. COLI [C] . I. Rocha, E.C. Ferreira Proceedings of the 15th IFAC World Congress: International Federation of Automatic Control . 2003

机译：基于模型的大肠杆菌重组蛋白生产过程中乙酸盐浓度的自适应控制
5. Susceptibility of the Fall Armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) to Bacillus thuringiensis (Bt) Proteins and Synthetic Insecticides from Different Corn Production Systems in Mexico and Puerto Rico [D] . Gutierrez-Moreno, Rebeca Adriana 2017

机译：秋季粘虫，Spodoptera frugiperda（J. E. Smith）（鳞翅目：夜蛾科）对苏云金芽胞杆菌（Bt）蛋白和来自墨西哥和波多黎各不同玉米生产系统的合成杀虫剂的敏感性
6. The E. coli Single Protein Production (cSPP) System for Production and Structural Analysis of Membrane Proteins [O] . Lili Mao, S. Thangminlal Vaiphei, Tsutomu Shimazu, -1

机译：膜蛋白的生产和结构分析的大肠杆菌单蛋白质生产（CSPP）系统
7. The Use of the Condensed Single Protein Production System for Isotope-Labeled Outer Membrane Proteins, OmpA and OmpX in E. coli [O] . S. Thangminlal Vaiphei, Yuefeng Tang, Gaetano T. Montelione, 2010

机译：在大肠杆菌中使用浓缩单蛋白质生产系统的同位素标记的外膜蛋白，OMPA和OMPX
8. Structure Analysis of OmpC, One of the Major Proteins in the Outer Membrane of E. Coli, by High Resolution Electron Microscopy [R] . Chang, C. F. 1983

机译：高分辨率电子显微镜观察大肠杆菌外膜中主要蛋白OmpC的结构

The E. coli single protein production system for production and structural analysis of membrane proteins

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