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首页> 外文期刊>Journal of Tissue Engineering >Cancer Tissue Engineering: A Novel 3D Polystyrene Scaffold for In Vitro Isolation and Amplification of Lymphoma Cancer Cells from Heterogeneous Cell Mixtures
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Cancer Tissue Engineering: A Novel 3D Polystyrene Scaffold for In Vitro Isolation and Amplification of Lymphoma Cancer Cells from Heterogeneous Cell Mixtures

机译：癌症组织工程：一种新型3D聚苯乙烯支架，用于从异种细胞混合物中体外分离和扩增淋巴瘤癌细胞。

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摘要

Isolation and amplification of primary lymphoma cells in vitro setting is technically and biologically challenging task. To optimize culture environment and mimic in vivo conditions, lymphoma cell lines were used as a test case and were grown in 3-dimension (3D) using a novel 3D tissue culture polystyrene scaffold with neonatal stromal cells to represent a lymphoma microenvironment. In this model, the cell proliferation was enhanced more than 200-fold or 20,000% neoplastic surplus in 7 days when less than 1% lymphoma cells were cocultured with 100-fold excess of neonatal stroma cells, representing 3.2-fold higher proliferative rate than 2D coculture model. The lymphoma cells grew and aggregated to form clusters during 3D coculture and did not maintained the parental phenotype to grow in single-cell suspension. The cluster size was over 5-fold bigger in the 3D coculture by day 4 than 2D coculture system and contained less than 0.00001% of neonatal fibroblast trace. This preliminary data indicate that novel 3D scaffold geometry and coculturing environment can be customized to amplify primary cancer cells from blood or tissues related to hematological cancer and subsequently used for personalized drug screening procedures.
机译：原发性淋巴瘤细胞的体外分离和扩增是一项技术和生物学挑战性任务。为了优化培养环境并模拟体内条件，将淋巴瘤细胞系用作测试案例，并使用带有新生基质细胞的新型3D组织培养聚苯乙烯支架以3维（3D）生长，以代表淋巴瘤微环境。在该模型中，当少于1％的淋巴瘤细胞与100倍过量的新生基质细胞共培养时，细胞增殖在7天之内可增强200倍以上或20,000％的肿瘤剩余，这比2D的增殖率高3.2倍共培养模型。淋巴瘤细胞在3D共培养过程中生长并聚集形成簇，并且在单细胞悬液中并未保持亲本表型生长。到第4天，在3D共培养中的簇大小比2D共培养系统大5倍以上，并且包含少于0.00001％的新生儿成纤维细胞痕迹。该初步数据表明，可以定制新颖的3D支架几何形状和共培养环境，以从血液或与血液癌相关的组织中扩增原代癌细胞，并随后用于个性化药物筛选程序。

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