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首页> 外文期刊>Journal of Traditional Chinese Medical Sciences >Cinobufagin, a bufadienolide from traditional Chinese medicine Bufo bufo gargarizans CANTOR, inhibits PC3 cell growth in?vitro and in?vivo
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Cinobufagin, a bufadienolide from traditional Chinese medicine Bufo bufo gargarizans CANTOR, inhibits PC3 cell growth in?vitro and in?vivo

机译：Cinobufagin是来自中药 Bufo bufo gargarizans CANTOR的丁二烯内酯，可抑制PC3细胞的体外生长和。 vivo

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ObjectiveTo explore the effects of cinobufagin (CBF), an active component of toad venom (Bufo bufo gargarizansCANTOR), on the proliferation and apoptosis of PC3 human prostate cancer cellsin?vitroand preliminarily investigate the mechanism of CBF in suppressing tumor cell growthin?vivo.MethodsThe effect of CBF on PC3 cells proliferation was detected using MTT assay. The morphological changes of PC3 cells were observed under an optical microscope. Colony formation assays were used to observe the CBF effect on colony formation by PC3 cells. PC3 cell apoptosis after treatment with CBF for 48?hours was monitored using flow cytometry. Furthermore, the effect of CBF on the expression of myeloid cell leukemia-1 (MCL-1) and related apoptotic proteins was examined using western blotting. A xenograft model was established in BALB/c nude mice to evaluate the effect of CBF on prostate cancerin?vivo.ResultsThe MTT assay results illustrated that PC3 cell proliferation was inhibitedin?vitroby CBF in a concentration- and time-dependent manner. Compared with the control group findings, CBF significantly inhibited the formation of PC3 cells (P?=?.005). Flow cytometry revealed that after treatment with 50?nM CBF for 48?hours, the apoptotic rate of PC3 cells was 41.97 (5.16)%, indicating that CBF could significantly induce its apoptosis (P?=?.003). In addition, optical and fluorescence microscopy uncovered remarkable inhibition of cell proliferation accompanied by morphologic changes. The western blotting result indicated that CBF obviously downregulated the expression of the anti-apoptotic protein MCL-1. Most importantly, CBF reduced the carcinogenicity of PC3 xenografts in nude mice.ConclusionCBF can inhibit the growth of PC3 cells bothin?vitroandin?vivoand induce apoptosis of tumor cells. The corresponding mechanism may be correlated with the activation of caspase family proteins via MCL-1.
机译：目的探讨蟾毒的活性成分西蟾蜍精（CBF）对体外PC3人前列腺癌细胞的增殖和凋亡的影响，并初步探讨CBF抑制体内肿瘤细胞生长的机制。 MTT法检测CBF对PC3细胞增殖的影响。在光学显微镜下观察PC3细胞的形态变化。集落形成测定用于观察CBF对PC3细胞集落形成的影响。使用流式细胞仪监测CBF处理48小时后PC3细胞的凋亡。此外，使用蛋白质印迹法检查了CBF对髓样细胞白血病1（MCL-1）和相关凋亡蛋白表达的影响。在BALB / c裸鼠体内建立了异种移植模型，以评价CBF对前列腺癌的体内杀伤作用。结果MTT分析表明，CBF对PC3细胞的增殖具有浓度和时间依赖性的抑制作用。与对照组相比，CBF显着抑制了PC3细胞的形成（P == 0.005）。流式细胞仪检测结果表明，用50？nM CBF处理48？h后，PC3细胞的凋亡率为41.97（5.16）％，表明CBF可以显着诱导其凋亡（P？=？0.003）。此外，光学和荧光显微镜发现伴随细胞形态改变的细胞增殖受到显着抑制。 Western印迹结果表明，CBF明显下调了抗凋亡蛋白MCL-1的表达。最重要的是，CBF降低了PC3异种移植裸鼠的致癌性。结论CBF可以抑制PC3细胞在体外和体内的生长，并诱导肿瘤细胞凋亡。相应的机制可能与通过MCL-1激活caspase家族蛋白有关。

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