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首页> 外文期刊>Journal of Young Pharmacists >Phytochemical Analysis, Antioxidant Properties and Brine Shrimp Lethality of Unripe Fruits of Solanum viarum
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Phytochemical Analysis, Antioxidant Properties and Brine Shrimp Lethality of Unripe Fruits of Solanum viarum

机译：茄子未成熟果实的植物化学分析，抗氧化特性和卤虾对虾的致死性

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Objective: The aim of the study was evaluate the tannin content, antioxidant activity and the lethality of unripe fruits extract of Solanum viarum Dunal (Solanaceae) against brine shrimps. Methods: The tested samples for tannins content were water, ethanol:water (1:3) and acetone:water (1:3) extract. The unripe fruits powder of S. viarum was used to extraction in a Soxhlet apparatus for 2 hr. Cytotoxicity was screened using Brine Shrimp Lethality Test and aqueous extract. Results: The results demonstrated that the yield of tannins for the acetone:water extraction was substantially higher than that typical yields obtained by ethanol:water or water extraction [31.10% for Total Solids (%), 14.99% for Stiasny’s Index (%), 3.99% for Condensed Tannins in the Extract (%) and 1.09% for Condensed Tannins in the Unripe Fruits (%). The antioxidant activity was 61.12±12.42 % compared to ascorbic acid (100%) and polyphenol concentration was 39.33±4.45 mg of tannic acid/g of extract obtained from the tannic acid calibration curve y = 0.0128x - 0.0015 (R2 = 0.9988). Flavonoid concentration was 89.36 ± 11.6 mg of quercetin/g of extract obtained by quercetin calibration curve y=0.0153x+0.0109, R2=0.9827. The results demonstrated that the positive control (potassium dichromate) hatching success was significantly higher than the S. viarum extract (P50 was 66.01 μg/ml for the water extract of unripe fruit of S. viarum and in accord to Clarkson’s toxicity criterion, LC50< 100 μg/mL are considered highly toxic for aqueous extract. Conclusion: The unripe fruit extract of S. viarum demonstrated low percentage in phenolic content, but significant antioxidant activity and phenolic and flavonoid concentrations. S. viarum unripe fruits extract is toxic to Artemia salina .
机译：目的：研究茄子茄（Solanaceae）未成熟果实提取物对盐水虾的单宁含量，抗氧化活性和致死性。方法：测试的单宁含量样品为水，乙醇：水（1：3）和丙酮：水（1：3）提取物。将未成熟的葡萄球菌的水果粉末在索氏提取器中提取2小时。使用盐水虾致死性试验和水提取物筛选细胞毒性。结果：结果表明，丙酮：水提取的单宁产率明显高于乙醇：水或水提取的典型产率[总固体含量（％）为31.10％，施蒂斯尼指数（％）为14.99％，提取物中浓缩单宁的含量为3.99％（％），未成熟果实中浓缩的单宁含量为1.09％（％）。与抗坏血酸（100％）相比，抗氧化剂活性为61.12±12.42％，多酚浓度为39.33±4.45 mg鞣酸/克从鞣酸校准曲线y提取的提取物y = 0.0128x-0.0015（R 2 = 0.9988）。通过槲皮素校正曲线y = 0.0153x + 0.0109，R 2 = 0.9827获得的提取物中的类黄酮浓度为89.36±11.6 mg槲皮素/ g。结果表明，阳性对照（重铬酸钾）的孵化成功率明显高于S. viarum提取物（P50 为66.01μg/ ml），这符合Clarkson的毒性。结论：LC 50 <100μg/ mL被认为对水提取物具有高毒性结论：未成熟的葡萄球菌果实提取物的酚含量低，但具有明显的抗氧化活性以及酚类和类黄酮的浓度S. viarum未成熟的水果提取物对卤虫卤虫有毒。

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