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首页> 外文期刊>BMC Genomics >De novo transcriptome assembly of drought tolerant CAM plants, Agave deserti and Agave tequilana
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De novo transcriptome assembly of drought tolerant CAM plants, Agave deserti and Agave tequilana

机译：耐旱的CAM植物，龙舌兰和龙舌兰的从头转录组组装

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Background Agaves are succulent monocotyledonous plants native to xeric environments of North America. Because of their adaptations to their environment, including crassulacean acid metabolism (CAM, a water-efficient form of photosynthesis), and existing technologies for ethanol production, agaves have gained attention both as potential lignocellulosic bioenergy feedstocks and models for exploring plant responses to abiotic stress. However, the lack of comprehensive Agave sequence datasets limits the scope of investigations into the molecular-genetic basis of Agave traits. Results Here, we present comprehensive, high quality de novo transcriptome assemblies of two Agave species, A. tequilana and A. deserti, built from short-read RNA-seq data. Our analyses support completeness and accuracy of the de novo transcriptome assemblies, with each species having a minimum of approximately 35,000 protein-coding genes. Comparison of agave proteomes to those of additional plant species identifies biological functions of gene families displaying sequence divergence in agave species. Additionally, a focus on the transcriptomics of the A. deserti juvenile leaf confirms evolutionary conservation of monocotyledonous leaf physiology and development along the proximal-distal axis. Conclusions Our work presents a comprehensive transcriptome resource for two Agave species and provides insight into their biology and physiology. These resources are a foundation for further investigation of agave biology and their improvement for bioenergy development.
机译：背景龙舌兰是北美干燥环境原生的肉质单子叶植物。由于龙舌兰对环境的适应性，包括十字绣果酸代谢（一种高效节水的光合作用形式）和现有的乙醇生产技术，龙舌兰作为潜在的木质纤维素生物能源原料和探索植物对非生物胁迫反应的模型而受到关注。 。然而，缺乏全面的龙舌兰序列数据集限制了对龙舌兰性状的分子遗传基础的研究范围。结果在这里，我们介绍了由短读RNA序列数据构建的两个龙舌兰物种A. tequilana和A. deserti的全面，高质量的从头转录组。我们的分析支持从头转录组装配的完整性和准确性，每个物种至少具有大约35,000个蛋白质编码基因。龙舌兰蛋白质组与其他植物物种的比较确定了显示龙舌兰物种序列差异的基因家族的生物学功能。此外，侧重于A. deserti幼叶的转录组学证实了单子叶植物叶片生理学的进化保守性和沿近端-远端轴的发育。结论我们的工作为两个龙舌兰物种提供了一个完整的转录组资源，并提供了对其生物学和生理学的深入了解。这些资源为进一步研究龙舌兰生物学及其对生物能源开发的改进奠定了基础。

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