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首页> 外文期刊>BMC Genomics >Effects of salinity on the transcriptome of growing maize leaf cells point at cell-age specificity in the involvement of the antioxidative response in cell growth restriction
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Effects of salinity on the transcriptome of growing maize leaf cells point at cell-age specificity in the involvement of the antioxidative response in cell growth restriction

机译：盐度对玉米叶片生长的转录组的影响表明细胞年龄的特异性参与了抗氧化反应参与细胞生长限制

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Background Salinity inhibits growth and development of most plants. The response to salinity is complex and varies between plant organs and stages of development. It involves challenges of ion toxicities and deficiencies as well as osmotic and oxidative stresses. The range of functions affected by the stress is reflected in elaborate changes to the transcriptome. The mechanisms involved in the developmental-stage specificity of the inhibitory responses are not fully understood. The present study took advantage of the well characterized developmental progression that exists along the maize leaf, for identification of salinity induced, developmentally-associated changes to the transcriptome. Differential subtraction screening was conducted for cells of two developmental stages: from the center of the growth zone where the expansion rate is highest, and from older cells at a more distal location of the growing zone where the expansion rate is lower and the salinity restrictive effects are more pronounced. Real-Time PCR analysis was used for validation of the expression of selected genes. Results The salinity-induced changes demonstrated an age-related response of the growing tissue, with elevation of salinity-damages with increased age. Growth reduction, similar to the elevation of percentage dry matter (%DM), and Na and Cl concentrations were more pronounced in the older cells. The differential subtraction screening identified genes encoding to proteins involved in antioxidant defense, electron transfer and energy, structural proteins, transcription factors and photosynthesis proteins. Of special interest is the higher induced expression of genes involved in antioxidant protection in the young compared to older cells, which was accompanied by suppressed levels of reactive oxygen species (H2O2 and O2-). This was coupled with heightened expression in the older cells of genes that enhance cell-wall rigidity, which points at reduced potential for cell expansion. Conclusions The results demonstrate a cell-age specificity in the salinity response of growing cells, and point at involvement of the antioxidative response in cell growth restriction. Processes involved in reactive oxygen species (ROS) scavenging are more pronounced in the young cells, while the higher growth sensitivity of older cells is suggested to involve effects on cell-wall rigidity and lower protein protection.
机译：背景盐度抑制大多数植物的生长和发育。对盐分的反应是复杂的，并且在植物器官和发育阶段之间变化。它涉及离子毒性和不足以及渗透和氧化应激的挑战。受转录影响的功能范围反映在转录组的精细变化中。抑制反应的发育阶段特异性涉及的机制尚未完全了解。本研究利用沿玉米叶片存在的特征明确的发育进程，以鉴定盐分诱导的转录组的发育相关的变化。对两个发育阶段的细胞进行了差减法筛选：从生长区中心的扩展率最高，到较老的细胞在生长区的远端，扩展率较低，并且限制了盐分作用更明显。实时PCR分析用于验证所选基因的表达。结果盐度引起的变化表明生长组织的衰老与年龄相关，随着年龄的增长，盐度损害增加。生长减少与干物质百分比（％DM）的增加类似，而Na和Cl浓度在较老的细胞中更为明显。差分减法筛选鉴定了编码涉及抗氧化防御，电子转移和能量的蛋白质，结构蛋白质，转录因子和光合作用蛋白质的基因。特别令人感兴趣的是，与年龄较大的细胞相比，年轻人中抗氧化保护相关基因的诱导表达更高，同时伴随着活性氧（H 2 O 2 和O 2 -）。这与在较老的细胞中增强基因表达有关，这些基因增强了细胞壁的刚性，从而降低了细胞扩增的潜力。结论结果表明，细胞年龄在生长细胞的盐度反应中具有特异性，并指出抗氧化反应与细胞生长受限有关。在年轻细胞中，参与清除活性氧（ROS）的过程更为明显，而较旧细胞的更高生长敏感性被认为涉及对细胞壁硬度和较低蛋白质保护的影响。

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