...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Genomics >Comparative genomics of the emerging human pathogen Photorhabdus asymbiotica with the insect pathogen Photorhabdus luminescens
【24h】

Comparative genomics of the emerging human pathogen Photorhabdus asymbiotica with the insect pathogen Photorhabdus luminescens

机译：新兴的人类病原体不对称光生细菌与昆虫病原体光致发光的比较基因组学

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background The Gram-negative bacterium Photorhabdus asymbiotica (Pa) has been recovered from human infections in both North America and Australia. Recently, Pa has been shown to have a nematode vector that can also infect insects, like its sister species the insect pathogen P. luminescens (Pl). To understand the relationship between pathogenicity to insects and humans in Photorhabdus we have sequenced the complete genome of Pa strain ATCC43949 from North America. This strain (formerly referred to as Xenorhabdus luminescens strain 2) was isolated in 1977 from the blood of an 80 year old female patient with endocarditis, in Maryland, USA. Here we compare the complete genome of Pa ATCC43949 with that of the previously sequenced insect pathogen P. luminescens strain TT01 which was isolated from its entomopathogenic nematode vector collected from soil in Trinidad and Tobago. Results We found that the human pathogen Pa had a smaller genome (5,064,808 bp) than that of the insect pathogen Pl (5,688,987 bp) but that each pathogen carries approximately one megabase of DNA that is unique to each strain. The reduced size of the Pa genome is associated with a smaller diversity in insecticidal genes such as those encoding the Toxin complexes (Tc's), Makes caterpillars floppy (Mcf) toxins and the Photorhabdus Virulence Cassettes (PVCs). The Pa genome, however, also shows the addition of a plasmid related to pMT1 from Yersinia pestis and several novel pathogenicity islands including a novel Type Three Secretion System (TTSS) encoding island. Together these data suggest that Pa may show virulence against man via the acquisition of the pMT1-like plasmid and specific effectors, such as SopB, that promote its persistence inside human macrophages. Interestingly the loss of insecticidal genes in Pa is not reflected by a loss of pathogenicity towards insects. Conclusion Our results suggest that North American isolates of Pa have acquired virulence against man via the acquisition of a plasmid and specific virulence factors with similarity to those shown to play roles in pathogenicity against humans in other bacteria.
机译：背景技术革兰氏阴性细菌非对称生光菌（Patrahabdus asymbiotica，Pa）已从北美和澳大利亚的人类感染中回收。最近，Pa已显示具有线虫载体，该线虫载体也可感染昆虫，如其姊妹物种昆虫病原体P. luminescens（P1）。为了了解Photorhabdus对昆虫和人类致病性之间的关系，我们对来自北美的Pa株ATCC43949的完整基因组进行了测序。该菌株（以前称为Xenorhabdus luminescens菌株2）于1977年从美国马里兰州一名80岁女性心内膜炎患者的血液中分离出来。在这里，我们将Pa ATCC43949的完整基因组与先前测序的昆虫病原体P. luminescens菌株TT01的完整基因组进行了比较，该菌株是从特立尼达和多巴哥土壤中收集的昆虫病原线虫载体中分离出来的。结果我们发现人类病原体Pa具有比昆虫病原体P1（5,688,987 bp）小的基因组（5,064,808 bp），但是每种病原体携带大约一个大碱基的DNA，这对于每个菌株而言都是唯一的。 Pa基因组的减少与杀虫基因的多样性较小有关，例如编码毒素复合物（Tc's），使毛毛虫软盘（Mcf）毒素和光生虫毒力盒（PVC）的那些。但是，Pa基因组还显示与鼠疫耶尔森氏菌pMT1相关的质粒和几个新的致病岛，包括一个新的三型分泌系统（TTSS）编码岛。这些数据加在一起，表明Pa可能通过收购pMT1样质粒和特定效应子（例如SopB）显示出对人的毒力，该效应子会促进其在人类巨噬细胞中的持久性。有趣的是，Pa中杀虫基因的丧失不能通过对昆虫的致病性丧失来反映。结论我们的结果表明，北美分离的Pa菌株通过获得质粒和特定的毒力因子而获得了对人的毒力，这些毒力因子与在其他细菌中对人的致病性起作用的因子相似。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号