...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Genomics >Genome-wide gene expression profiling of human mast cells stimulated by IgE or FcεRI-aggregation reveals a complex network of genes involved in inflammatory responses
【24h】

Genome-wide gene expression profiling of human mast cells stimulated by IgE or FcεRI-aggregation reveals a complex network of genes involved in inflammatory responses

机译：由IgE或FcεRI聚集刺激的人类肥大细胞的全基因组基因表达谱揭示了参与炎症反应的复杂基因网络

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background Mast cells are well established effectors of IgE-triggered allergic reactions and immune responses to parasitic infections. Recent studies indicate that mast cells may play roles in adaptive and innate immunity, suggesting an innovative view of the regulation of immune responses. Here, we profiled the transcriptome of human mast cells sensitized with IgE alone, or stimulated by FcεRI aggregation. Results Our data show that among 8,793 genes examined, 559 genes are differentially regulated in stimulated mast cells when compared with resting/unstimulated mast cells. The major functional categories of upregulated genes include cytokines, chemokines, and other genes involved in innate and adaptive immune-responses. We observed the increased expression of over 63 gene-transcripts following IgE-sensitization alone. Our data was validated using Real-Time-PCR; ELISA and western blot. We confirmed that IgE alone does not trigger mast cell-immediate responses, such as calcium signals, degranulation or protein-phosphorylation. Conclusion This report represents a substantial advance in our understanding of the genome wide effects triggered by "passive sensitization" or active stimulation of human mast cells, supporting mast cells' potential involvement in a wide range of inflammatory responses.
机译：背景肥大细胞是IgE触发的过敏反应和对寄生虫感染的免疫反应的公认效应子。最近的研究表明，肥大细胞可能在适应性免疫和先天性免疫中发挥作用，这提示了对免疫应答调节的创新观点。在这里，我们介绍了人类肥大细胞的转录组，仅用IgE敏感，或受FcεRI聚集刺激。结果我们的数据表明，与静息/未刺激的肥大细胞相比，受刺激的肥大细胞中检测到的8,793个基因中有559个基因受到差异调节。上调基因的主要功能类别包括细胞因子，趋化因子和其他与先天和适应性免疫反应有关的基因。我们观察到仅IgE致敏后超过63种基因转录物的表达增加。我们的数据已通过Real-Time-PCR验证; ELISA和蛋白质印迹。我们证实仅IgE不会触发肥大细胞直接反应，例如钙信号，脱颗粒或蛋白质磷酸化。结论该报告代表了我们对“被动敏化”或主动刺激人类肥大细胞引发的全基因组效应的理解的实质性进展，支持了肥大细胞潜在参与多种炎症反应。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号