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首页> 外文期刊>BMC Genomics >Genomic regions responsible for seminal and crown root lengths identified by 2D & 3D root system image analysis
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Genomic regions responsible for seminal and crown root lengths identified by 2D & 3D root system image analysis

机译：通过2D和3D根系图像分析确定负责精根和冠根长度的基因组区域

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Genetic improvement of root system architecture is a promising approach for improved uptake of water and mineral nutrients distributed unevenly in the soil. To identify genomic regions associated with the length of different root types in rice, we quantified root system architecture in a set of 26 chromosome segment substitution lines derived from a cross between lowland indica rice, IR64, and upland tropical japonica rice, Kinandang Patong, (IK-CSSLs), using 2D & 3D root phenotyping platforms. Lengths of seminal and crown roots in the IK-CSSLs grown under hydroponic conditions were measured by 2D image analysis (RootReader2D). Twelve CSSLs showed significantly longer seminal root length than the recurrent parent IR64. Of these, 8 CSSLs also exhibited longer total length of the three longest crown roots compared to IR64. Three-dimensional image analysis (RootReader3D) for these CSSLs grown in gellan gum revealed that only one CSSL, SL1003, showed significantly longer total root length than IR64. To characterize the root morphology of SL1003 under soil conditions, SL1003 was grown in Turface, a soil-like growth media, and roots were quantified using RootReader3D. SL1003 had larger total root length and increased total crown root length than did IR64, although its seminal root length was similar to that of IR64. The larger TRL in SL1003 may be due to increased crown root length. SL1003 carries an introgression from Kinandang Patong on the long arm of chromosome 1 in the genetic background of IR64. We conclude that this region harbors a QTL controlling crown root elongation.
机译：根系结构的遗传改良是改善土壤中不均匀分布的水分和矿质养分吸收的一种有前途的方法。为了确定与水稻中不同根系类型的长度相关的基因组区域，我们在一组26条染色体片段替代系中对根系体系结构进行了定量，这些系来源于低地in稻IR64和高地热带粳稻Kinandang Patong（ IK-CSSLs），使用2D和3D根表型平台。通过2D图像分析（RootReader2D）测量在水培条件下生长的IK-CSSLs中的精浆和冠状根的长度。十二个CSSLs的精子根长度明显比轮回亲本IR64长。其中，与IR64相比，8个CSSL的三个最长冠根的总长度也更长。这些在结冷胶中生长的CSSL的三维图像分析（RootReader3D）显示，只有一种CSSL SL1003的总根长比IR64长得多。为了表征SL1003在土壤条件下的根系形态，将SL1003在类似土壤的生长介质Turface中生长，并使用RootReader3D定量根系。 SL1003具有比IR64更大的总根长和增加的总冠根长，尽管其精根长与IR64相似。 SL1003中的TRL较大可能是由于冠根长度增加所致。 SL1003在IR64的遗传背景中从1号染色体长臂上的Kinandang Patong渗入。我们得出结论，该区域具有控制冠根伸长的QTL。

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